Kwak Jung Hyun, Kim Yoonji, Ryu Soo In, Lee Minho, Lee Hyo-Jeong, Lim Yong Pyo, Paik Jean Kyung
Department of Food and Nutrition Eulji University Seongnam Korea.
Nutrition Team Wonkwang University Sanbon Hospital Gunpo Korea.
Food Sci Nutr. 2020 Feb 24;8(4):1898-1903. doi: 10.1002/fsn3.1472. eCollection 2020 Apr.
A chronic inflammatory environment facilitates tumor growth and proliferation. Fruits and vegetables are important sources of anthocyanins, polyphenols, and other biologically active substances that can favorably affect the pathogenesis of cancer. The objective of the study was to investigate the anti-inflammatory effects of Red Chinese cabbage (RC) and mixture of commercial Red Chinese cabbage leaves and Aronia fruits (ARC) in LPS-stimulated RAW 264.7 cells. The RAW 264.7 cells were cultured and measured the cytotoxicity by using an MTT assay. The inflammatory markers, such as nitrite, IL-6, and TNF-alpha expression, were evaluated using ELISA, and protein expression of inflammatory markers like iNOS and COX-2 was analyzed using Western blot. MTT assays showed that pretreatment of RAW 264.7 cells with RC and ARC did not change cell growth or cytotoxicity. We also found that ARC extracts reduced inflammation-related biomarker (TNF-a, IL-6, and NO) production and gene expression (iNOS, COX-2). Our results suggested that ARC has good anti-inflammatory properties compared with RC that maybe used as potential nutrients for treating inflammatory diseases.
慢性炎症环境促进肿瘤生长和增殖。水果和蔬菜是花青素、多酚及其他可对癌症发病机制产生有利影响的生物活性物质的重要来源。本研究的目的是调查红白菜（RC）以及市售红白菜叶与野樱莓果实混合物（ARC）对脂多糖刺激的RAW 264.7细胞的抗炎作用。培养RAW 264.7细胞并使用MTT法测定细胞毒性。使用酶联免疫吸附测定法评估亚硝酸盐、白细胞介素-6和肿瘤坏死因子-α等炎症标志物的表达，并使用蛋白质印迹法分析诱导型一氧化氮合酶和环氧化酶-2等炎症标志物的蛋白质表达。MTT分析表明，用RC和ARC预处理RAW 264.7细胞不会改变细胞生长或细胞毒性。我们还发现，ARC提取物可减少炎症相关生物标志物（肿瘤坏死因子-α、白细胞介素-6和一氧化氮）的产生及基因表达（诱导型一氧化氮合酶、环氧化酶-2）。我们的结果表明，与RC相比，ARC具有良好的抗炎特性，可能用作治疗炎症性疾病的潜在营养物质。
