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胡椒和槟榔水提取物对小鼠成纤维细胞、人口腔普通上皮 1 号和人口腔鳞状细胞癌细胞系的细胞毒性作用。

Cytotoxic Effects of Betel Quid and Areca Nut Aqueous Extracts on Mouse Fibroblast, Human Mouth-Ordinary-Epithelium 1 and Human Oral Squamous Cell Carcinoma Cell Lines.

机构信息

School of Dental Sciences, Universiti Sains Malaysia, Health Campus, 16150 Kubang Kerian, Kelantan, Malaysia.

Graduate School of Medical and Dental Sciences, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima, Japan.

出版信息

Asian Pac J Cancer Prev. 2020 Apr 1;21(4):1005-1009. doi: 10.31557/APJCP.2020.21.4.1005.

DOI:10.31557/APJCP.2020.21.4.1005
PMID:32334462
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7445995/
Abstract

BACKGROUND

Betel quid chewing is more common among the older generation in rural areas of Malaysia. Oral cancer in Asia has been associated with the habit of chewing betel quid and areca nut.

OBJECTIVE

This study aims to investigate the cytotoxic effects of betel quid and areca nut extracts on the fibroblast (L929), mouth-ordinary-epithelium 1 (MOE1) and oral squamous cell carcinoma (HSC-2) cell lines.

METHODS

L929, MOE1 and HSC-2 cells were treated with 0.1, 0.2 and 0.4 g/ml of betel quid and areca nut extracts for 24, 48 and 72 h. MTT assay was performed to assess the cell viability.

RESULTS

Both extracts, regardless of concentration, significantly reduced the cell viability of L929 compared with the control (P<0.05). Cell viability of MOE1 was significantly enhanced by all betel quid concentrations compared with the control (P<0.05). By contrast, 0.4 g/ml of areca nut extract significantly reduced the cell viability of MOE1 at 48 and 72 h of incubation. Cell viability of HSC-2 was significantly lowered by all areca nut extracts, but 0.4 g/ml of betel quid significantly increased the cell viability of HSC-2 (P<0.05).

CONCLUSION

Areca nut extract is cytotoxic to L929 and HSC-2, whereas the lower concentrations of areca nut extract significantly increased the cell viability of MOE1 compared to the higher concentration and control group. Although betel quid extract is cytotoxic to L929, the same effect is not observed in MOE1 and HSC-2 cell lines. Further investigations are needed to clarify the mechanism of action.
.

摘要

背景

咀嚼槟榔在马来西亚农村的老年人群中更为常见。亚洲地区的口腔癌与咀嚼槟榔和槟榔果的习惯有关。

目的

本研究旨在探讨槟榔和槟榔果提取物对成纤维细胞（L929）、口腔正常上皮细胞 1（MOE1）和口腔鳞状细胞癌细胞（HSC-2）的细胞毒性作用。

方法

用 0.1、0.2 和 0.4 g/ml 的槟榔和槟榔果提取物处理 L929、MOE1 和 HSC-2 细胞 24、48 和 72 h。采用 MTT 法评估细胞活力。

结果

与对照组相比，两种提取物（无论浓度如何）均显著降低了 L929 的细胞活力（P<0.05）。与对照组相比，所有槟榔浓度均显著增强了 MOE1 的细胞活力（P<0.05）。相比之下，0.4 g/ml 的槟榔果提取物在 48 和 72 h 孵育时显著降低了 MOE1 的细胞活力。所有槟榔果提取物均显著降低了 HSC-2 的细胞活力，但 0.4 g/ml 的槟榔提取物显著增加了 HSC-2 的细胞活力（P<0.05）。

结论

槟榔果提取物对 L929 和 HSC-2 具有细胞毒性，而较低浓度的槟榔果提取物与较高浓度和对照组相比，显著增加了 MOE1 的细胞活力。虽然槟榔提取物对 L929 具有细胞毒性，但在 MOE1 和 HSC-2 细胞系中未观察到相同的作用。需要进一步的研究来阐明其作用机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb38/7445995/634ec64b36fc/APJCP-21-1005-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb38/7445995/605542308b6b/APJCP-21-1005-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb38/7445995/242d8c9852ef/APJCP-21-1005-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb38/7445995/634ec64b36fc/APJCP-21-1005-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb38/7445995/605542308b6b/APJCP-21-1005-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb38/7445995/242d8c9852ef/APJCP-21-1005-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb38/7445995/634ec64b36fc/APJCP-21-1005-g003.jpg

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