Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran; Department of Tissue Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran; Department of Biomaterials, Tissue Engineering and Nanotechnology, School of Advanced Technologies in Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Biomaterials, Tissue Engineering and Nanotechnology, School of Advanced Technologies in Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Int J Biol Macromol. 2020 Sep 15;159:154-173. doi: 10.1016/j.ijbiomac.2020.05.073. Epub 2020 May 13.
Lignin displays attractive properties in peripheral nerve applications. Here, aligned polycaprolactone (PCL) fibers with various percentages of lignin nanoparticles were fabricated using the electrospinning method. The morphologies, contact angles, mechanical properties, in vitro degradation, and water uptake of the PCL/lignin fibers were characterized. Cell viability and adhesion of PC12 and human adipose-derived stem cells (hADSCs) were studied employing MTT assay and SEM, respectively. SEM, immunocytochemistry, and Real-Time PCR were utilized to characterize neural differentiation and neurite length of PC12 and hADSCs. To further study on lignin effect on nerve regeneration, in vivo studies were performed. The results indicated that all nanocomposite fibers were smooth and bead-free. With increasing the lignin content, the water contact angle decreased while in vitro degradation, water uptake, and Young's modulus increased compared to the PCL fibers. Cell viability, and differentiation along with neurite length extension were promoted by increasing lignin content. The neural markers expression for differentiated cells were upregulated by the increase of lignin percent. In vivo investigation also demonstrates that sample groups incorporating 15% lignin nanoparticles showed better regeneration among others. Therefore, PCL with 15% of lignin nanoparticles shows great potential to be applied for nerve regeneration.
木质素在周围神经应用中表现出诱人的特性。在这里，使用静电纺丝法制备了具有不同百分比木质素纳米粒子的定向聚己内酯（PCL）纤维。对 PCL/木质素纤维的形态、接触角、力学性能、体外降解和吸水率进行了表征。通过 MTT 测定法和 SEM 分别研究了 PC12 和人脂肪源性干细胞（hADSCs）的细胞活力和黏附。SEM、免疫细胞化学和实时 PCR 用于表征 PC12 和 hADSCs 的神经分化和神经突长度。为了进一步研究木质素对神经再生的影响，进行了体内研究。结果表明，所有纳米复合材料纤维均光滑无珠。与 PCL 纤维相比，随着木质素含量的增加，水接触角降低，而体外降解、吸水率和杨氏模量增加。随着木质素含量的增加，细胞活力和分化以及神经突长度的延长得到促进。随着木质素百分比的增加，分化细胞的神经标志物表达上调。体内研究还表明，含有 15%木质素纳米粒子的样品组在其他组中表现出更好的再生。因此，含有 15%木质素纳米粒子的 PCL 具有很大的潜力应用于神经再生。
