Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, Utrecht 3584 CT, The Netherlands.
Department of Microbiology, University of Helsinki, P.O. Box 56, Viikinkaari 9, Helsinki, Finland.
Biotechnol Adv. 2020 Nov 1;43:107551. doi: 10.1016/j.biotechadv.2020.107551. Epub 2020 May 11.
Filamentous fungi are important industrial cell factories used for the production of a wide range of enzymes and metabolites. Their primary metabolism is a significant source of industrially important compounds, as well as of monomeric building blocks for the production of secondary metabolites and extracellular enzymes. Therefore, large efforts have been made towards the development of suitable strains for the industrial scale production of primary metabolites. Over the last decades, metabolic engineering of primary metabolism has become a powerful tool to enhance production of both primary and secondary metabolites. This review summarises the different metabolic engineering methods that have been applied to rationally improve the production of industrially relevant primary metabolites in filamentous fungi, and discusses related challenges and future perspectives.
丝状真菌是重要的工业细胞工厂，用于生产广泛的酶和代谢物。它们的初级代谢是工业上重要化合物的重要来源，也是生产次生代谢物和细胞外酶的单体建筑块的重要来源。因此，人们做出了巨大的努力来开发适合大规模生产初级代谢物的菌株。在过去的几十年中，初级代谢物的代谢工程已经成为一种强大的工具，可以提高初级和次生代谢物的生产。本文综述了应用于合理提高丝状真菌中工业相关初级代谢物生产的不同代谢工程方法，并讨论了相关的挑战和未来展望。
