Chemical Process Engineering, RWTH Aachen University, Forckenbeckstr. 51, 52074, Aachen, Germany.
DWI Leibnitz-Institute for Interactive Materials, Forckenbeckstr. 50, 52074, Aachen, Germany.
Angew Chem Int Ed Engl. 2020 Sep 7;59(37):16047-16053. doi: 10.1002/anie.202003287. Epub 2020 Jun 25.
Metal-organic frameworks (MOFs) are suitable enzyme immobilization matrices. Reported here is the in situ biomineralization of glucose oxidase (GOD) into MOF crystals (ZIF-8) by interfacial crystallization. This method is effective for the selective coating of porous polyethersulfone microfiltration hollow fibers on the shell side in a straightforward one-step process. MOF layers with a thickness of 8 μm were synthesized, and fluorescence microscopy and a colorimetric protein assay revealed the successful inclusion of GOD into the ZIF-8 layer with an enzyme concentration of 29±3 μg cm . Enzymatic activity tests revealed that 50 % of the enzyme activity is preserved. Continuous enzymatic reactions, by the permeation of β-d-glucose through the GOD@ZIF-8 membranes, showed a 50 % increased activity compared to batch experiments, emphasizing the importance of the convective transport of educts and products to and from the enzymatic active centers.
金属-有机骨架（MOFs）是合适的酶固定化基质。本文报道了通过界面结晶将葡萄糖氧化酶（GOD）原位生物矿化为 MOF 晶体（ZIF-8）。该方法可在一步法中有效地对壳侧的多孔聚醚砜微滤中空纤维进行选择性涂覆。合成了厚度为 8 μm 的 MOF 层，荧光显微镜和比色法蛋白测定表明 GOD 成功地包含在 ZIF-8 层中，酶浓度为 29±3 μg·cm 。酶活性测试表明保留了 50%的酶活性。通过 GOD@ZIF-8 膜渗透β-D-葡萄糖进行连续酶反应，与分批实验相比，活性增加了 50%，这强调了反应物和产物向和从酶活性中心的对流传输的重要性。
