Darabian Bita, Bagheri Hamed, Mohammadi Soheila
Faculty of Interdisciplinary Science and Technology, Tarbiat Modares University, Tehran, Iran.
Pharmaceutical Science Research Center, Health Institute, Kermanshah University of Medical Science, Kermanshah, Iran.
Prog Biomater. 2020 Jun;9(1-2):45-64. doi: 10.1007/s40204-020-00131-6. Epub 2020 May 30.
Wound is among the most common injuries. A suitable wound dressing has a significant effect on the healing process. In this study, a porous wound dressing was prepared using poly (lactic acid) (PLA) and two plasticizers, polyethylene glycol (PEG) and triacetin (TA), through solvent casting method. For antibacterial activities, metronidazole was incorporated in the structure. The morphology was investigated by scanning electron microscopy (SEM). In addition, the effect of plasticizers ratio on porosity growth was evaluated. It was also observed that each had a unique effect on the structure's porosity. The mechanical properties confirmed the effect of both plasticizers on increasing polymer softness and flexibility, and the most similar formulations to human skin in terms of mechanical properties were introduced. According to the results, TA had stronger effect on mechanical properties. The differential scanning calorimetry (DSC) showed the effect of increasing plasticizer concentration on crystalline structure and T reduction of PLA. The water contact angle measurement showed that both plasticizers enhanced hydrophilic characteristics of PLA, and this effect was weaker in PEG-containing formulations. The in vitro degradation study showed biodegradability, as a desirable property in wound dressing. Results suggested that higher degradation can be obtained by both plasticizers at the same time. The results also showed that PEG was more effective in enhancing water absorbency. In vitro drug release study indicated an explosive release and the highest amount was 85% over 186 h. The antibacterial activity test confirmed the effectiveness of the drug in preventing bacterial growth in the drug-containing formulations, while it showed the antibacterial property of TA. MTT assay was performed and the cellular toxicity of the formulations was checked and those that revealed the least toxicity were introduced.
伤口是最常见的损伤之一。合适的伤口敷料对愈合过程有显著影响。在本研究中，通过溶剂浇铸法，使用聚乳酸（PLA）以及两种增塑剂聚乙二醇（PEG）和甘油三乙酸酯（TA）制备了一种多孔伤口敷料。为了具备抗菌活性，在结构中加入了甲硝唑。通过扫描电子显微镜（SEM）研究其形态。此外，评估了增塑剂比例对孔隙率增长的影响。还观察到每种增塑剂对结构的孔隙率都有独特的影响。力学性能证实了两种增塑剂对提高聚合物柔软度和柔韧性的作用，并介绍了在力学性能方面与人体皮肤最相似的配方。结果表明，TA对力学性能的影响更强。差示扫描量热法（DSC）显示了增塑剂浓度增加对PLA结晶结构和熔点降低的影响。水接触角测量表明，两种增塑剂都增强了PLA的亲水性，且在含PEG的配方中这种效果较弱。体外降解研究表明其具有生物降解性，这是伤口敷料所需的特性。结果表明，两种增塑剂同时使用可获得更高的降解率。结果还表明，PEG在提高吸水性方面更有效。体外药物释放研究表明有爆发性释放，在186小时内最高释放量为85%。抗菌活性测试证实了药物在含药配方中对防止细菌生长的有效性，同时显示了TA的抗菌性能。进行了MTT测定，检查了配方的细胞毒性，并介绍了毒性最小的配方。
