Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, China.
The Save Sight Institute, Faculty of Medicine and Health, the University of Sydney, Sydney, NSW 2000, Australia.
Trends Biotechnol. 2021 Jan;39(1):43-58. doi: 10.1016/j.tibtech.2020.05.006. Epub 2020 Jun 3.
Revealing fine-scale cellular heterogeneity among spatial context and the functional and structural foundations of tissue architecture is fundamental within biological research and pharmacology. Unlike traditional approaches involving single molecules or bulk omics, cutting-edge, spatially resolved transcriptomics techniques offer near-single-cell or even subcellular resolution within tissues. Massive information across higher dimensions along with position-coordinating labels can better map the whole 3D transcriptional landscape of tissues. In this review, we focus on developments and strategies in spatially resolved transcriptomics, compare the cell and gene throughput and spatial resolution in detail for existing methods, and highlight the enormous potential in biomedical research.
揭示空间背景下的精细细胞异质性以及组织架构的功能和结构基础，是生物学研究和药理学中的基本问题。与涉及单个分子或整体组学的传统方法不同，前沿的、空间分辨的转录组学技术可在组织内实现近乎单细胞甚至亚细胞分辨率。高维海量信息以及位置协调标签可更好地绘制组织的整个 3D 转录图谱。在这篇综述中，我们重点介绍空间分辨转录组学的发展和策略，详细比较现有方法的细胞和基因通量和空间分辨率，并强调其在生物医学研究中的巨大潜力。
