银杏内生真菌次生代谢产物的抗癌活性。
Anti-cervical cancer activity of secondary metabolites of endophytic fungi from Ginkgo biloba.
机构信息
Shandong Key Laboratory in University of Functional Bioresource Utilization, College of Life Science, Dezhou University, Dezhou, Shandong, China.
Shandong Provincial Key Laboratory of Biophysics, Dezhou University, Dezhou, Shandong, China.
出版信息
Cancer Biomark. 2020;28(3):371-379. doi: 10.3233/CBM-190462.
OBJECTIVES
The purpose of this study was to isolate the secondary metabolites of endophytic fungi from Ginkgo biloba (SMEFGB) and investigate their anti-cervical cancer activity.
METHODS
SMEFGB were cultured. The secondary metabolites of endophytic fungi was extracted, purified and identified. The effects of secondary metabolites on proliferation, apoptosis and migration of human cervical cancer HeLa cells were determined. In addition, the effects of SMEFGB on growth of Hela implanted tumor in mice were investigated.
RESULTS
In 9 stains of endophytic fungi successfully isolated from the leaves of Ginkgo biloba, the stain J-1, J-2 and J-3 could produce podophyllotoxin. These 3 stains were identified by molecular biology. The secondary metabolites of stain J-1, J-2 and J-3 markedly inhibited the proliferation of HeLa cells, promoted their apoptosis and blocked their migration. In addition, the secondary metabolites of stain J-1, J-2 and J-3 significantly attenuated the growth of HeLa implanted tumor in mice.
CONCLUSIONS
Our results indicated that SMEFGB had obvious anti-cervical cancer activity in vitro and in vivo.
目的
本研究旨在从银杏（SMEFGB）中分离内生真菌的次生代谢产物，并研究其抗宫颈癌活性。
方法
培养 SMEFGB。提取、纯化和鉴定内生真菌的次生代谢产物。测定次生代谢产物对人宫颈癌 HeLa 细胞增殖、凋亡和迁移的影响。此外，还研究了 SMEFGB 对荷瘤小鼠生长的影响。
结果
从银杏叶片中成功分离出 9 株内生真菌，其中菌株 J-1、J-2 和 J-3 可产生鬼臼毒素。这 3 株菌通过分子生物学鉴定。菌株 J-1、J-2 和 J-3 的次生代谢产物显著抑制 HeLa 细胞的增殖，促进其凋亡并阻断其迁移。此外，菌株 J-1、J-2 和 J-3 的次生代谢产物显著抑制 HeLa 植入瘤在小鼠体内的生长。
结论
本研究结果表明，SMEFGB 具有明显的体内外抗宫颈癌活性。
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