Paun Gabriela, Neagu Elena, Albu Camelia, Savin Simona, Radu Gabriel Lucian
National Institute of Research and Development for Biological Sciences, Bucharest 6 060031, Romania.
ACS Omega. 2020 May 22;5(22):13014-13022. doi: 10.1021/acsomega.0c00929. eCollection 2020 Jun 9.
This study was focused on the phytochemical composition and biological activities of and polyphenolic-rich extracts obtained by nanofiltration. The high-performance liquid chromatography-mass spectrometry analysis showed that chlorogenic acid and rosmarinic acid were the main phenolic acids in both extracts. The main flavonoid compound from extracts is luteolin, whereas rutin and isoquercitrin are the main flavonoids in . polyphenolic-rich extract had the highest α-amylase (from hog pancreas) inhibitory activity (IC = 1.30 ± 0.06 μg/mL) and α-glucosidase (from ) inhibitory activity (IC = 92.18 ± 1.92 μg/mL). However, both extracts presented a significant α-glucosidase inhibitory activity. Furthermore, the hyaluronidase inhibition of polyphenolic-rich extracts also proved to be stronger (IC = 11.8 ± 0.1 μg/mL for and 36.5 ± 0.2 μg/mL for ), but there was moderate or low lipoxygenase inhibition. The studies on the fibroblast cell line demonstrated that both and polyphenolic-rich extracts possess the cytotoxic effect at a concentration higher than 500 μg/mL. The experimental data suggest that both extracts are promising candidates for the development of natural antidiabetic and anti-inflammatory food supplements.
本研究聚焦于通过纳滤获得的[具体物质1]和[具体物质2]富含多酚提取物的植物化学成分及生物活性。高效液相色谱 - 质谱分析表明，绿原酸和迷迭香酸是两种提取物中的主要酚酸。[具体物质1]提取物中的主要黄酮类化合物是木犀草素，而芦丁和异槲皮苷是[具体物质2]中的主要黄酮类化合物。[具体物质1]富含多酚提取物具有最高的α - 淀粉酶（来自猪胰腺）抑制活性（IC = 1.30 ± 0.06 μg/mL）和α - 葡萄糖苷酶（来自[具体来源]）抑制活性（IC = 92.18 ± 1.92 μg/mL）。然而，两种提取物均呈现出显著的α - 葡萄糖苷酶抑制活性。此外，富含多酚提取物对透明质酸酶的抑制作用也更强（[具体物质1]的IC = 11.8 ± 0.1 μg/mL，[具体物质2]的IC = 36.5 ± 0.2 μg/mL），但对脂氧合酶的抑制作用为中度或低度。对成纤维细胞系的研究表明，[具体物质1]和[具体物质2]富含多酚提取物在浓度高于500 μg/mL时均具有细胞毒性作用。实验数据表明，这两种提取物有望用于开发天然抗糖尿病和抗炎食品补充剂。
