Arslan Kalbim, Özant Ali, Özçay Necdet, Çalış İhsan, Besim Hasan
Yakın Doğu Üniversitesi Tıp Fakültesi, Genel Cerrahi Anabilim Dalı, Lefkoşa, Kıbrıs.
Yakın Doğu Üniversitesi Eczacılık Fakültesi, Lefkoşa, Kıbrıs.
Turk J Surg. 2019 Mar 1;35(1):30-34. doi: 10.5578/turkjsurg.4170. eCollection 2019 Mar.
Hydatid disease is a parasitic disease caused by Echinococcus granulosus and is still endemic in many parts of the world. Scolicidal solutions are generally used in any type of intervention, either surgical or percutaneous, to neutralize the cyst contents, although completeness of their effect is obscure and solid evidence is scarce. On the other hand, the use of these scolicidal solutions is not devoid of complications and many serious complications such as caustic sclerosing cholangitis may be seen in relation with their usage. Recent investigations proved protoscolicidal properties of olive leaf extract although the active ingredient has not been attributed to any component. The aim of this experimental study was to isolate oleuropein and test for in vitro protoscolicidal activity.
Oleuropein, a phenolic compound found in olive leaves, is extracted and prepared in different concentrations. Echinococcal cyst containing livers of sheep are obtained from the government slaughterhouse. Cysts were punctured and live protoscolex suspensions were prepared under aseptic conditions. Different concentrations of oleuropein solutions were prepared and protoscolicidal property is analyzed and compared with positive and negative controls for different exposure times.
Oleuropein 2% concentration was found to be protoscolicidal in all exposure times starting from 5 minutes.
2% oleuropein is a powerful, natural protoscolicidal agent which should be evaluated clinically before its application in routine treatment practice.
包虫病是由细粒棘球绦虫引起的一种寄生虫病，在世界许多地区仍然流行。杀头节剂通常用于任何类型的干预，无论是手术还是经皮干预，以中和囊肿内容物，尽管其效果的完整性尚不清楚且确凿证据稀少。另一方面，使用这些杀头节剂并非没有并发症，并且与它们的使用相关可能会出现许多严重并发症，如腐蚀性硬化性胆管炎。最近的研究证明了橄榄叶提取物的原头节杀灭特性，尽管尚未将活性成分归因于任何成分。本实验研究的目的是分离橄榄苦苷并测试其体外原头节杀灭活性。
从橄榄叶中提取并制备不同浓度的橄榄苦苷，一种酚类化合物。从政府屠宰场获取含有羊肝包虫囊肿。对囊肿进行穿刺，并在无菌条件下制备活的原头节悬液。制备不同浓度的橄榄苦苷溶液，并分析其原头节杀灭特性，并与不同暴露时间的阳性和阴性对照进行比较。
发现2%浓度的橄榄苦苷在从5分钟开始的所有暴露时间内都具有原头节杀灭作用。
2%的橄榄苦苷是一种强大的天然原头节杀灭剂，在其应用于常规治疗实践之前应进行临床评估。
