Programa de Pos-Graduacao em Ciencias Medicas, Universidade Federal Fluminense, Niteroi, RJ, BR.
Division of Gynecologic Oncology, Department of Obstetrics and Gynecology and Reproductive Biology, New England Trophoblastic Disease Center, Brigham and Women's Hospital, Dana-Farber Cancer Institute, Boston, MA, USA.
Clinics (Sao Paulo). 2020;75:e1724. doi: 10.6061/clinics/2020/e1724. Epub 2020 Jul 6.
This study aimed to evaluate the potential relationship between oxidative stress, dietary intake, and serum levels of antioxidants in patients with a complete hydatidiform mole (CHM) compared with controls.
This was an observational cross-sectional study conducted in Rio de Janeiro, Brazil. A total of 140 women were enrolled in this study and divided into four groups: 43 patients with CHM, 33 women who had had an abortion, 32 healthy pregnant women, and 32 healthy non-pregnant women. All participants underwent blood sampling, assessment using a semiquantitative food frequency questionnaire, and anthropometric measurement. Blood samples were collected after overnight fasting (10-12 h). Vitamin levels (A, C, and E) were determined by ultra-performance liquid chromatography, and gamma-glutamyl transferase levels were assessed using an automated quantitative analysis system (Dimension®, Siemens).
Although all groups showed sufficient serum vitamin A and E levels, the participants had inadequate dietary intake of these vitamins. Conversely, all groups had an insufficient serum level of vitamin C, despite adequate intake. The gamma-glutamyl transferase values did not differ significantly among the groups. However, elevated serum levels of this enzyme were observed in several patients.
All groups exhibited high levels of oxidative stress, as evaluated by gamma-glutamyl transferase levels, and had inadequate intake of antioxidant vitamins. Therefore, the high exposure to oxidative stress found in our study, even in healthy pregnant and non-pregnant women, may increase the incidence of CHM in this region.
本研究旨在评估完全性葡萄胎（CHM）患者与对照组相比，氧化应激、饮食摄入和血清抗氧化剂水平之间的潜在关系。
这是一项在巴西里约热内卢进行的观察性横断面研究。共有 140 名女性参与了这项研究，分为四组：43 名 CHM 患者、33 名流产妇女、32 名健康孕妇和 32 名健康未怀孕妇女。所有参与者均接受了采血、半定量食物频率问卷评估和人体测量。采血在禁食 10-12 小时后进行。采用超高效液相色谱法测定维生素水平（A、C 和 E），采用自动定量分析系统（Dimension®，西门子）评估γ-谷氨酰转移酶水平。
尽管所有组的血清维生素 A 和 E 水平都足够，但这些维生素的饮食摄入量不足。相反，所有组的血清维生素 C 水平不足，尽管摄入量充足。各组的γ-谷氨酰转移酶值没有显著差异。然而，在一些患者中观察到该酶的血清水平升高。
所有组的γ-谷氨酰转移酶水平均显示出高水平的氧化应激，且抗氧化维生素摄入不足。因此，即使在健康的孕妇和未怀孕的妇女中，我们研究中发现的高氧化应激暴露可能会增加该地区 CHM 的发病率。
