Wu Shengru, Chen Xiaodong, Li Taohuan, Ren Hao, Zheng Lixin, Yang Xiaojun
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi China.
Center for Translational Microbiome Research, Department of Molecular, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.
J Anim Sci Biotechnol. 2020 Jul 6;11:73. doi: 10.1186/s40104-020-00480-z. eCollection 2020.
Glucose oxidase (GOD), an aerobic dehydrogenase, has been used as an antibiotic substitute in feed. A study was conducted to evaluate the differential effects of 2 different GODs fermented by or on caecal microbiota and to further illuminate the potential roles of changes in the gut microbiota in regulating the growth performance and meat quality of broiler chickens.
A total of 420 one-day-old healthy Arbor Acres broilers were randomly assigned to 4 treatments: the control group, the antibiotic growth promoter (AGP) supplementation group, and the GOD-A and GOD-P (GODs produced by and , respectively) groups. As a result, supplementation with GOD produced by could significantly improve the average daily weight gain and average daily feed intake of broilers before 21 days of age by significantly increasing the enzymatic activities of jejunal amylase and those of ileal amylase, chymotrypsin, and lipase in 21-day-old broilers and could increase the enzymatic activities of duodenal amylase, jejunal amylase and lipase, and ileal chymotrypsin and lipase in 42-day-old broilers. Meanwhile, compared with AGP treatment, supplementation with GOD produced by significantly decreased the L value of 21-day-old broilers and the ΔpH and L* value of 42-day-old broilers, while supplementation with GOD produced by significantly increased the pH value of 21-day-old and 42-day-old broilers by reducing plasma malondialdehyde content. By using 16S rRNA sequencing, we found that the beneficial bacteria and microbiota in broilers were not disturbed but were improved by GOD supplementation compared with ADP treatment, including the genera and and the species , , and , which were positively related to the improved intestinal digestive enzymatic activities, growth performance, and meat quality of broilers.
The altered gut microbiota induced by supplementation with glucose oxidase produced by mediate better regulatory effects on the meat quality and growth performance of broilers than that induced by supplementation with glucose oxidase produced by .
葡萄糖氧化酶（GOD）是一种需氧脱氢酶，已被用作饲料中的抗生素替代品。本研究旨在评估由[具体发酵方式1]或[具体发酵方式2]发酵的两种不同GOD对盲肠微生物群的差异影响，并进一步阐明肠道微生物群变化在调节肉鸡生长性能和肉质方面的潜在作用。
总共420只1日龄健康艾维茵肉鸡被随机分为4组：对照组、抗生素生长促进剂（AGP）添加组以及GOD - A组和GOD - P组（分别由[具体发酵方式1]和[具体发酵方式2]产生的GOD）。结果表明，添加由[具体发酵方式1]产生的GOD可显著提高21日龄前肉鸡的平均日增重和平均日采食量，这是通过显著提高21日龄肉鸡空肠淀粉酶以及42日龄肉鸡十二指肠淀粉酶、空肠淀粉酶和脂肪酶、回肠胰凝乳蛋白酶和脂肪酶的酶活性实现的。同时，与AGP处理相比，添加由[具体发酵方式1]产生的GOD显著降低了21日龄肉鸡的L值以及42日龄肉鸡的ΔpH和L*值，而添加由[具体发酵方式2]产生的GOD通过降低血浆丙二醛含量显著提高了21日龄和42日龄肉鸡的pH值。通过16S rRNA测序，我们发现与AGP处理相比，肉鸡中的有益细菌和微生物群未受干扰，反而因添加GOD而得到改善，包括[具体菌属1]、[具体菌属2]等菌属以及[具体菌种1]、[具体菌种2]和[具体菌种3]等菌种，它们与肉鸡肠道消化酶活性、生长性能和肉质的改善呈正相关。
添加由[具体发酵方式1]产生的葡萄糖氧化酶诱导的肠道微生物群变化对肉鸡的肉质和生长性能的调节作用优于添加由[具体发酵方式2]产生的葡萄糖氧化酶所诱导的变化。
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