Meng Yong, Huo Haonan, Zhang Yang, Bai Shiping, Wang Ruisheng, Zhang Keying, Ding Xuemei, Wang Jianping, Zeng Qiufeng, Peng Huanwei, Xuan Yue
Feed Engineering Research Centre of Sichuan Province, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China.
Mianyang Habio Bioengineering Co., Ltd., Mianyang 610000, China.
Animals (Basel). 2021 Oct 8;11(10):2909. doi: 10.3390/ani11102909.
This study aimed to investigate the effects of glucose oxidase (GOD) supplementation on growth performance, apparent ileal digestibility (AID) of nutrients, intestinal morphology, and short-chain fatty acids (SCFAs) and microbiota in the ileum of broilers. Six hundred 1-day-old male broilers were randomly allotted to four groups of 10 replicates each with 15 birds per replicate cage. The four treatments included the basal diet without antibiotics (Control) and the basal diet supplemented with 250, 500, or 1000 U GOD/kg diet (E250, E500 or E1000). The samples of different intestinal segments, ileal mucosa, and ileal digesta were collected on d 42. Dietary GOD supplementation did not affect daily bodyweight gain (DBWG) and the ratio of feed consumption and bodyweight gain (FCR) during d 1-21 ( > 0.05); however, the E250 treatment increased DBWG ( = 0.03) during d 22-42 as compared to control. Dietary GOD supplementation increased the AIDs of arginine, isoleucine, lysine, methionine, threonine, cysteine, serine, and tyrosine ( < 0.05), while no significant difference was observed among the GOD added groups. The E250 treatment increased the villus height of the jejunum and ileum. The concentrations of secreted immunoglobulin A (sIgA) in ileal mucosa and the contents of acetic acid and butyric acid in ileal digesta were higher in the E250 group than in the control ( < 0.05), whereas no significant differences among E500, E1000, and control groups. The E250 treatment increased the richness of ileal microbiota, but E500 and E100 treatment did not significantly affect it. Dietary E250 treatment increased the relative abundance of phylum and genus, while it decreased the relative abundance of genus ( < 0.05). Phylum only colonized in the ileal digesta of E500 treated broilers and E500 and E1000 did not affect the relative abundance of phylum and and genera as compared to control. These results suggested that dietary supplementation of 250 U GOD/kg diet improves the growth performance of broilers during d 22-42, which might be associated with the alteration of the intestinal morphology, SCFAs composition, and ileal microbiota composition.
本研究旨在探讨添加葡萄糖氧化酶（GOD）对肉鸡生长性能、养分表观回肠消化率（AID）、肠道形态、短链脂肪酸（SCFAs）以及回肠微生物群的影响。600只1日龄雄性肉鸡被随机分为四组，每组10个重复，每个重复笼养15只鸡。四种处理包括不添加抗生素的基础日粮（对照组）以及添加250、500或1000 U GOD/kg日粮的基础日粮（E250、E500或E1000）。在第42天采集不同肠段、回肠黏膜和回肠食糜的样本。日粮中添加GOD在第1至21天对日均体重增加（DBWG）和采食量与体重增加之比（FCR）没有影响（P>0.05）；然而，与对照组相比，E250处理在第22至42天提高了DBWG（P = 0.03）。日粮中添加GOD提高了精氨酸、异亮氨酸、赖氨酸、蛋氨酸、苏氨酸、半胱氨酸、丝氨酸和酪氨酸的AID（P<0.
