Mohamed Erieg A, Bordean Despina M, Radulov Isidora, Moruzi Răzvan F, Hulea Călin I, Orășan Sergiu A, Dumitrescu Eugenia, Muselin Florin, Herman Hildegard, Brezovan Diana, Hermenean Anca, Cristina Romeo T
Banat University of Agriculture and Veterinary Medicine "King Michael I of Romania" from Timisoara, 119 Calea Aradului, Timisoara 300645, Romania.
"Vasile Goldis" Western University Arad, Revoluției Blvd. No. 94, Arad, Romania.
Evid Based Complement Alternat Med. 2020 Jun 22;2020:1736803. doi: 10.1155/2020/1736803. eCollection 2020.
Medications to reduce oxidative stress are preventing cellular damage associated with hyperlipidemia. In this regard, statins (e.g., atorvastatin) act primarily by decrease in low-density lipoprotein- but, in the last decade, hepatotoxicity, associated with liver injuries in the next months after treatments' initiation, was reported. In this case, associated phytotherapy can be a solution.
To investigate the antioxidant potential and response to free radicals, in the case of hyperlipidemic rats treated with atorvastatin. Sea buckthorn () and a grape extract (antioxivita) efficiency in the oxidative stress were investigated, also being ascertained the rats' organs cytoarchitecture.
Eighty-four hyperlipidemic Wistar rats were divided into seven groups and orally treated as follows: ATS, atorvastatin (20 mg/kg·bw); ATS + Hr, atorvastatin + ; ATS + Aox, atorvastatin + grape extract; Hr, ; and Aox, grape extract (both as 100 mg/kg·bw). HFD and Control received high fat diet and normal fodder only. After two and six months, respectively, rats were euthanized and the heart, liver, and kidneys were gathered. The tissue samples were prepared by homogenization of 0.5 g tissue, in ethanol, kept for 48 hours at 4°C-10°C and then filtered, in order to assess organs' cytoarchitecture and the TAC's values (by using cupric ion reducing antioxidant capacity (CUPRAC) assay). The test tubes were incubated, at room temperature, for 30 minutes, and then analyzed using a spectrophotometer at 450-650 nm.
The statistics (ANOVA) revealed that sea buckthorn diminished notably ( < 0.001) the oxidative stress in the heart, liver, and kidney. After six months, the TAC's reduced levels for the heart were significant ( < 0.001) in ATS + Aox. In the case of histology, the liver's cytoarchitecture in ATS revealed abnormal cytoarchitecture. In ATS + Hr, ATS + Aox, Hr, and Aox, cell regeneration improved in different stages, especially for ATS + Hr and ATS + Aox, in comparison with HFD, which exhibited fat degeneration. Kidney's cytoarchitecture revealed cellular healing, especially in ATS + Hr and ATS + Aox.
用于减轻氧化应激的药物可预防与高脂血症相关的细胞损伤。在这方面，他汀类药物（如阿托伐他汀）主要通过降低低密度脂蛋白起作用，但在过去十年中，有报道称在开始治疗后的接下来几个月里会出现与肝损伤相关的肝毒性。在这种情况下，联合植物疗法可能是一种解决方案。
研究用阿托伐他汀治疗的高脂血症大鼠的抗氧化潜力和对自由基的反应。研究了沙棘（）和葡萄提取物（抗氧化维他）在氧化应激方面的功效，并确定了大鼠器官的细胞结构。
将84只高脂血症Wistar大鼠分为7组，进行如下口服治疗：ATS组，阿托伐他汀（20毫克/千克体重）；ATS + Hr组，阿托伐他汀 + ；ATS + Aox组，阿托伐他汀 + 葡萄提取物；Hr组，；Aox组，葡萄提取物（均为100毫克/千克体重）。HFD组和对照组分别只接受高脂饮食和正常饲料。分别在两个月和六个月后，对大鼠实施安乐死并采集心脏、肝脏和肾脏。通过将0.5克组织在乙醇中匀浆制备组织样本，在4°C - 10°C下保存48小时，然后过滤，以评估器官的细胞结构和总抗氧化能力（TAC）值（通过使用铜离子还原抗氧化能力（CUPRAC）测定法）。将试管在室温下孵育30分钟，然后使用分光光度计在450 - 650纳米处进行分析。
统计学分析（方差分析）显示，沙棘显著降低了心脏、肝脏和肾脏中的氧化应激（<0.001）。六个月后，ATS + Aox组心脏的TAC降低水平显著（<0.001）。在组织学方面，ATS组肝脏的细胞结构显示异常细胞结构。在ATS + Hr组、ATS + Aox组、Hr组和Aox组中，细胞再生在不同阶段有所改善，特别是ATS + Hr组和ATS + Aox组，与表现出脂肪变性的HFD组相比。肾脏的细胞结构显示细胞愈合，特别是在ATS + Hr组和ATS + Aox组中。
