Antifungal Nafuredin and Epithiodiketopiperazine Derivatives From the Mangrove-Derived Fungus D13.

A new polyketide derivative, nafuredin C (), a novel heterocyclic dipeptide, trichodermamide G (), together with four known biogenetically related compounds nafuredin A (), trichodermamide A (), aspergillazin A (), and peniisocoumarin H (), were isolated from the mangrove-derived fungus D13. Their structures, including their absolute configurations, were determined by spectroscopic analysis and time-dependent density functional theory-electronic circular dichroism (ECD) calculations. Trichodermamide G was found to be a novel epithiodiketopiperazine derivative with an unprecedented cyclic system containing a sulfur bridge, and nafuredin C represented the third nafuredin derivative of these homologous compounds. The new compound nafuredin C exhibited obvious antifungal activity against with a minimum inhibitory concentration (MIC) of 8.63 μM, which is on the same order of magnitude as the positive control carbendazim (MIC = 3.27 μM).