Department of Clinical Laboratory Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, People's Republic of China.
School of Life Science and Technology, Shanghai Tech University, Shanghai, 201210, People's Republic of China.
J Nanobiotechnology. 2020 Jul 20;18(1):100. doi: 10.1186/s12951-020-00656-9.
Plant-derived extracellular vesicles (PDEVs) have great potential for clinical applications. Ultracentrifugation, considered the gold standard method for the preparation of PDEVs, is efficacious but time-consuming and highly instrument-dependent. Thus, a rapid and handy method is needed to facilitate the basic researches and clinical applications of PDEVs.
In this study, we combined electrophoretic technique with 300 kDa cut-off dialysis bag (named ELD) for the isolation of PDEVs, which was time-saving and needed no special equipment. Using ELD, lemon derived extracellular vesicles (LDEVs) could be isolated from lemon juice. Nanoparticle tracking analysis and transmission electron microscopy confirmed that the method separated intact vesicles with a similar size and number to the standard method-ultracentrifugation. LDEVs caused the gastric cancer cell cycle S-phase arrest and induced cell apoptosis. The anticancer activities of LDEVs on gastric cancer cells were mediated by the generation of reactive oxygen species. In addition, LDEVs were safe and could be remained in gastrointestinal organs.
ELD was an efficient method for the isolation of LDEVs, and could be carried out in any routine biological laboratory as no special equipment needed. LDEVs exerted anticancer activities on gastric cancer, indicating the great potentials for clinical application as edible chemotherapeutics delivery vehicle.
植物来源的细胞外囊泡 (PDEV) 在临床应用中具有巨大的潜力。超速离心法被认为是制备 PDEV 的金标准方法，但该方法有效但耗时且高度依赖仪器。因此，需要一种快速便捷的方法来促进 PDEV 的基础研究和临床应用。
在这项研究中，我们将电泳技术与 300 kDa 截止透析袋（命名为ELD）结合用于分离 PDEV，该方法省时且不需要特殊设备。使用ELD，可以从柠檬汁中分离出柠檬衍生的细胞外囊泡（LDEV）。纳米颗粒跟踪分析和透射电子显微镜证实，该方法分离出的囊泡完整，大小和数量与超速离心的标准方法相似。LDEV 导致胃癌细胞周期 S 期停滞并诱导细胞凋亡。LDEV 对胃癌细胞的抗癌活性是通过产生活性氧介导的。此外，LDEV 是安全的，可以保留在胃肠道器官中。
ELD 是一种分离 LDEV 的有效方法，可以在任何常规的生物学实验室中进行，因为不需要特殊设备。LDEV 对胃癌具有抗癌活性，表明其作为可食用化疗药物递送载体在临床应用中的巨大潜力。
