Ruckman Leigh A, Petry Amy L, Gould Stacie A, Patience John F
Department of Animal Science, Iowa State University, Ames, IA.
Transl Anim Sci. 2020 Jun 26;4(3):txaa095. doi: 10.1093/tas/txaa095. eCollection 2020 Jul.
The objective of this experiment was to compare the effects of spray-dried plasma protein (SDPP) and dried egg protein (DEP), without (AB-) or with (AB+) in-feed antibiotics, on growth performance and markers of intestinal health in nursery pigs raised in commercial conditions. This 42-d experiment utilized 1,230 pigs (4.93 ± 0.04 kg body weight; approximately 15-18 d of age). Pigs were randomly assigned to one of six dietary treatments that were arranged as a 2 × 3 factorial of in-feed antibiotics (AB- vs. AB+) and a specialty protein additive (none [CON], porcine SDPP, or DEP). Diets were fed in four phases with phases 3 and 4 as a common diet across all treatments. Specialty protein additives were fed in phases 1 (0-13 d; 3% SDPP, and 0.20% DEP) and 2 (13-26 d; 2% SDPP, and 0.10% DEP). Antibiotics were fed in phases 1-3 (662 mg chlortetracycline [CTC]/kg, 28 mg carbadox/kg, and 441 mg CTC/kg, respectively). Ileal tissue and blood samples were collected from 48 pigs (8 per treatment) on d 20. Data were analyzed using PROC MIXED of SAS (9.4) with pen as the experimental unit; protein additives, antibiotics, and their interaction were fixed effects and block was a random effect. The pigs experienced naturally occurring health challenges in weeks 2 and 4. In the AB- diets, SDPP and DEP increased average daily gain (ADG; = 0.036) and average daily feed intake (ADFI; = 0.040) compared to CON; in the AB+ diets, neither SDPP nor DEP increased ADG or ADFI compared to CON but SDPP did increase these parameters over DEP. The SDPP and DEP diets decreased the number of individual medical treatments compared to CON ( = 0.001). The AB+ increased ileal mucosal interleukin (IL)-1 receptor antagonist ( = 0.017). Feeding DEP reduced the concentration of mucosal IL-1β compared to CON, but not SDPP ( = 0.022). There was a trend for SDPP and DEP to increase villus height:crypt depth compared to CON ( = 0.066). Neither antibiotics or protein additive affected serum malondialdehyde concentration or ileal mRNA abundance of or , , or ( > 0.10). In conclusion, SDPP and DEP improved growth performance of weaned pigs in the absence of antibiotics but neither improved growth compared to CON when feeding standard antibiotic levels. The specialty proteins had a positive effect on health; specialty proteins and antibiotics were able to modulate some markers of intestinal inflammation and morphology.
本实验的目的是比较喷雾干燥血浆蛋白（SDPP）和干燥鸡蛋蛋白（DEP）在不添加（AB-）或添加（AB+）饲料抗生素的情况下，对在商业条件下饲养的保育猪生长性能和肠道健康指标的影响。这项为期42天的实验使用了1230头猪（体重4.93±0.04千克；约15 - 18日龄）。猪被随机分配到六种日粮处理之一，这些处理按饲料抗生素（AB-与AB+）和特殊蛋白质添加剂（无[CON]、猪源SDPP或DEP）的2×3析因设计安排。日粮分四个阶段饲喂，第3阶段和第4阶段为所有处理的通用日粮。特殊蛋白质添加剂在第1阶段（0 - 13天；3% SDPP和0.20% DEP）和第2阶段（13 - 26天；2% SDPP和0.10% DEP）饲喂。抗生素在第1 - 3阶段饲喂（分别为662毫克金霉素[CTC]/千克、28毫克卡巴氧/千克和441毫克CTC/千克）。在第20天从48头猪（每个处理8头）采集回肠组织和血液样本。数据使用SAS（9.4）的PROC MIXED程序进行分析，以栏为实验单位；蛋白质添加剂、抗生素及其相互作用为固定效应，区组为随机效应。猪在第2周和第4周经历了自然发生的健康挑战。在AB-日粮中，与CON相比，SDPP和DEP提高了平均日增重（ADG；P = 0.036）和平均日采食量（ADFI；P = 0.040）；在AB+日粮中，与CON相比，SDPP和DEP均未提高ADG或ADFI，但SDPP相对于DEP确实提高了这些参数。与CON相比，SDPP和DEP日粮减少了个体药物治疗的次数（P = 0.001）。AB+增加了回肠黏膜白细胞介素（IL）-1受体拮抗剂（P = 0.017）。与CON相比，饲喂DEP降低了黏膜IL-1β的浓度，但SDPP没有（P = 0.022）。与CON相比，SDPP和DEP有增加绒毛高度与隐窝深度比值的趋势（P = 0.066）。抗生素和蛋白质添加剂均未影响血清丙二醛浓度或回肠中TLR4、Occludin、ZO-1或Claudin-1的mRNA丰度（P>0.10）。总之，在不使用抗生素的情况下，SDPP和DEP改善了断奶仔猪的生长性能，但在饲喂标准抗生素水平时，与CON相比均未改善生长。特殊蛋白质对健康有积极影响；特殊蛋白质和抗生素能够调节一些肠道炎症和形态的指标。
