一种基于新型冠状病毒-1核糖体移码的药物筛选工具包。
A drug screening toolkit based on the -1 ribosomal frameshifting of SARS-CoV-2.
作者信息
Chen Yanqiong, Tao Huan, Shen Silan, Miao Zhiyong, Li Lili, Jia Yongqian, Zhang Hu, Bai Xiufeng, Fu Xinyuan
机构信息
Laboratory of Human Disease and Immunotherapies, West China Hospital, Sichuan University, Chengdu, China.
National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan Province, China.
出版信息
Heliyon. 2020 Aug;6(8):e04793. doi: 10.1016/j.heliyon.2020.e04793. Epub 2020 Aug 26.
The -1 ribosomal frameshifting is vital for the translation of the open reading frame (ORF)1b in SARS-CoV-2. The products of ORF1b participate in viral replication. Therefore, changing the frameshift frequency reduces the survival of the virus. This study aimed to successfully develop a toolkit for screening antiviral drugs. Finally, the FDA-approved drug library was screened, revealing that ivacaftor and (-)-Huperzine A worked well in changing the -1 ribosomal frameshifting of SARS-CoV-2 .
-1核糖体移码对于严重急性呼吸综合征冠状病毒2（SARS-CoV-2）中开放阅读框（ORF）1b的翻译至关重要。ORF1b的产物参与病毒复制。因此，改变移码频率会降低病毒的存活率。本研究旨在成功开发一种用于筛选抗病毒药物的工具包。最后，对美国食品药品监督管理局（FDA）批准的药物库进行了筛选，结果显示依伐卡托和（-）-石杉碱甲在改变SARS-CoV-2的-1核糖体移码方面效果良好。
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