Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
Mar Drugs. 2020 Aug 29;18(9):452. doi: 10.3390/md18090452.
Chemical examination from the cultured soft coral resulted in the isolation and structural identification of four new biscembranoidal metabolites, sardigitolides A-D (-), along with three previously isolated biscembranoids, sarcophytolide L (), glaucumolide A (), glaucumolide B (), and two known cembranoids ( and ). The chemical structures of all isolates were elucidated on the basis of 1D and 2D NMR spectroscopic analyses. Additionally, in order to discover bioactivity of marine natural products, - were examined in terms of their inhibitory potential against the upregulation of inflammatory factor production in lipopolysaccharide (LPS)-stimulated murine macrophage J774A.1 cells and their cytotoxicities against a limited panel of cancer cells. The anti-inflammatory results showed that at a concentration of 10 µg/mL, and inhibited the production of IL-1 to 68 ± 1 and 56 ± 1%, respectively, in LPS-stimulated murine macrophages J774A.1. Furthermore, sardigitolide B () displayed cytotoxicities toward MCF-7 and MDA-MB-231 cancer cell lines with the IC values of 9.6 ± 3.0 and 14.8 ± 4.0 µg/mL, respectively.
从培养的软珊瑚中进行的化学检查导致分离和结构鉴定了四种新的双半萜类代谢物，即 sardigitolides A-D(-)，以及三种先前分离的双半萜类化合物 sarcophytolide L()、glaucumolide A()、glaucumolide B()和两种已知的 cembranoids()和()。所有分离物的化学结构都是基于 1D 和 2D NMR 光谱分析来阐明的。此外，为了发现海洋天然产物的生物活性，对 - 进行了研究，以评估它们对脂多糖 (LPS) 刺激的小鼠巨噬细胞 J774A.1 细胞中炎症因子产生上调的抑制潜力及其对有限的癌细胞系的细胞毒性。抗炎结果表明，在 10 µg/mL 浓度下，和分别抑制 LPS 刺激的小鼠巨噬细胞 J774A.1 中 IL-1 的产生至 68 ± 1%和 56 ± 1%。此外，sardigitolide B()对 MCF-7 和 MDA-MB-231 癌细胞系具有细胞毒性，IC 值分别为 9.6 ± 3.0 和 14.8 ± 4.0 µg/mL。
Zotero 插件