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在依赖ATP的反应中，噬菌体λ阻遏物在体外的失活和蛋白水解切割。

Inactivation and proteolytic cleavage of phage lambda repressor in vitro in an ATP-dependent reaction.

作者信息

Roberts J W, Roberts C W, Mount D W

出版信息

Proc Natl Acad Sci U S A. 1977 Jun;74(6):2283-7. doi: 10.1073/pnas.74.6.2283.

DOI:10.1073/pnas.74.6.2283
PMID:329277
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC432154/
Abstract

We have reproduced in vitro the inactivation of bacteriophage lambda repressor that occurs in vivo when a lambda lysogen is treated with agents such as ultraviolet radiation that attack DNA. ATP and a divalent cation are required for the inactivation reaction. The ind- repressor is insensitive to the inactivation mechanism. A proteolytic cleavage of repressor accompanies inactivation in vitro, as it does in vivo.

摘要

我们在体外重现了噬菌体λ阻遏物的失活过程，该过程在体内当λ溶原菌受到诸如紫外线等攻击DNA的试剂处理时会发生。失活反应需要ATP和二价阳离子。ind-阻遏物对失活机制不敏感。体外失活过程中会伴随着阻遏物的蛋白水解切割，体内情况也是如此。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcdf/432154/dd2487b8c683/pnas00028-0104-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcdf/432154/dd2487b8c683/pnas00028-0104-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcdf/432154/dd2487b8c683/pnas00028-0104-a.jpg

相似文献

1
Inactivation and proteolytic cleavage of phage lambda repressor in vitro in an ATP-dependent reaction.在依赖ATP的反应中，噬菌体λ阻遏物在体外的失活和蛋白水解切割。
Proc Natl Acad Sci U S A. 1977 Jun;74(6):2283-7. doi: 10.1073/pnas.74.6.2283.
2
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Nature. 1980 Jan 3;283(5742):26-30. doi: 10.1038/283026a0.
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10
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Conditional induction of lambda-prophage in exrA mutants of Escherichia coli.大肠杆菌exrA突变体中λ原噬菌体的条件诱导
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5
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6
Prophage induction and cell division in E. coli. II. Linked (recA, zab) and unlinked (lex) suppressors of tif-1-mediated induction and filamentation.大肠杆菌中的原噬菌体诱导与细胞分裂。II. tif-1介导的诱导和丝状化的连锁（recA、zab）和非连锁（lex）抑制因子
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