MacKerrow S D, Merry J M, Hoeprich P D
J Clin Microbiol. 1987 May;25(5):885-8. doi: 10.1128/jcm.25.5.885-888.1987.
Synthetic amino acid medium for fungi (SAAMF) is a totally defined, nutritionally adequate, macromolecule-free culture medium for fungi that is buffered with an organic weak acid-weak base pair: 2-(N-morpholino)-propanesulfonic acid (MOPS) and 2-amino-2-(hydroxymethyl)-1,3-propanediol (Tris). In 1984, it was reported that MOPS-Tris in SAAMF antagonized the activity of flucytosine against Candida albicans (D. L. Calhoun and J. N. Galgiani, Antimicrob. Agents Chemother. 26:364-367, 1984). Accordingly, we evaluated the buffering capacity of seven synthetic organic buffers and monobasic potassium phosphate, both singly and in pairs, over the pH range 7.4 to 6.0. Of these buffers, MOPS, BES [N,N-bis(2-hydroxyethyl)-2-aminomethanesulfonic acid], a BES-MOPS combination, and KH2PO4 provided the best buffering. Growth of C. albicans, in unbuffered SAAMF was equivalent overall to that in SAAMF containing buffers, singly or in pairs. Twelve strains of C. albicans and five strains of Candida lusitaniae were tested for susceptibility to flucytosine in SAAMF, with and without buffers. In the presence of Tris, the geometric mean MICs were 6.5- and 3.6-fold higher, respectively, for C. albicans and C. lusitaniae. We recommend replacing Tris with the nonantagonistic MOPS.
真菌合成氨基酸培养基（SAAMF）是一种完全确定的、营养充足的、不含大分子的真菌培养基，它由有机弱酸 - 弱碱对缓冲：2 - （N - 吗啉代） - 丙烷磺酸（MOPS）和2 - 氨基 - 2 - （羟甲基） - 1,3 - 丙二醇（Tris）。1984年有报道称，SAAMF中的MOPS - Tris会拮抗氟胞嘧啶对白色念珠菌的活性（D. L. 卡尔霍恩和J. N. 加尔贾尼，《抗菌剂与化疗》26:364 - 367，1984）。因此，我们评估了七种合成有机缓冲剂和磷酸二氢钾在7.4至6.0的pH范围内单独及成对使用时的缓冲能力。在这些缓冲剂中，MOPS、BES [N，N - 双（2 - 羟乙基） - 2 - 氨基甲磺酸]、BES - MOPS组合以及KH2PO4提供了最佳缓冲效果。在未缓冲和含缓冲剂（单独或成对）的SAAMF中，白色念珠菌的生长总体相当。对12株白色念珠菌和5株葡萄牙念珠菌在含和不含缓冲剂的SAAMF中进行了氟胞嘧啶敏感性测试。在有Tris存在的情况下，白色念珠菌和葡萄牙念珠菌的几何平均MIC分别高出6.5倍和3.6倍。我们建议用无拮抗作用的MOPS取代Tris。
