Wallace Gregory Q, Masson Jean-François
Département de Chimie, Centre Québécois des Matériaux Fonctionnels (CQMF), and Regroupement Québécois des Matériaux de Pointe (RQMP), Université de Montréal, C.P. 6128 Succ. Centre-Ville, Montréal, QC H3C 3J7, Canada.
Analyst. 2020 Nov 9;145(22):7162-7185. doi: 10.1039/d0an01274b.
As surface-enhanced Raman scattering (SERS) continues to grow in popularity, more work needs to be done to evaluate its compatibility with a wider scope of applications. With such a strong emphasis on SERS being used for biosensing, it is important to examine how SERS is used in bioanalytical nanoscience, and more importantly, look towards where SERS is heading. For many, the initial steps involve demonstrating in vivo sensing by SERS using cultures of live cells. To further and better demonstrate the capabilities of SERS as a technique in bioanalytical nanoscience, it is necessary to transition away from studies involving single cells or small quantities of cells. This means working with tissue, typically as an ex vivo slice or a spheroid, before moving onto in vivo animal models. Although working with tissue as opposed to single cells introduces new challenges, the types of approaches developed for single cell studies serve as the foundation for the more complex biomaterials. The aim of this tutorial review is to better facilitate the transition from single cells to complex tissues by demonstrating the similarities in the methodologies that have been used and how to overcome some of the challenges of working with tissue. Specifically, we explore how three of the most common methods of working with nanoparticles and cells have been adapted and incorporated for experiments involving different types of tissues. Overall, this review highlights a variety of methods that can be readily implemented for those wishing to perform SERS measurements with or in complex tissues.
随着表面增强拉曼散射（SERS）的受欢迎程度不断提高，需要开展更多工作来评估其与更广泛应用范围的兼容性。鉴于如此强烈地强调SERS用于生物传感，研究SERS在生物分析纳米科学中的应用方式非常重要，更重要的是，要展望SERS的发展方向。对许多人来说，最初的步骤包括使用活细胞培养物通过SERS进行体内传感演示。为了进一步并更好地展示SERS作为生物分析纳米科学技术的能力，有必要从涉及单细胞或少量细胞的研究转向其他研究。这意味着在转向体内动物模型之前，要使用组织进行研究，通常是离体切片或球体。尽管与单细胞研究相比，使用组织会带来新的挑战，但为单细胞研究开发的方法类型为更复杂的生物材料研究奠定了基础。本教程综述的目的是通过展示已使用方法的相似性以及如何克服使用组织研究的一些挑战，更好地促进从单细胞研究向复杂组织研究的转变。具体而言，我们探讨了处理纳米颗粒和细胞的三种最常用方法是如何被调整并应用于涉及不同类型组织的实验中的。总体而言，本综述突出了多种方法，对于那些希望在复杂组织中或使用复杂组织进行SERS测量的人来说，这些方法可以很容易地实施。
