Mecê Pedro, Groux Kassandra, Scholler Jules, Thouvenin Olivier, Fink Mathias, Grieve Kate, Boccara Claude
Institut Langevin, ESPCI Paris, CNRS, PSL University, 1 rue Jussieu, 75005 Paris, France.
Institut de la Vision, Sorbonne Université, INSERM, CNRS, F-75012, Paris, France.
Biomed Opt Express. 2020 Aug 7;11(9):4928-4941. doi: 10.1364/BOE.400522. eCollection 2020 Sep 1.
Allying high-resolution with a large field-of-view (FOV) is of great importance in the fields of biology and medicine, but it is particularly challenging when imaging non-flat living samples such as the human retina. Indeed, high-resolution is normally achieved with adaptive optics (AO) and scanning methods, which considerably reduce the useful FOV and increase the system complexity. An alternative technique is time-domain full-field optical coherence tomography (FF-OCT), which has already shown its potential for high-resolution retinal imaging. Here, we introduce coherence gate shaping for FF-OCT, to optically shape the coherence gate geometry to match the sample curvature, thus achieving a larger FOV than previously possible. Using this instrument, we obtained high-resolution images of living human photoreceptors close to the foveal center without AO and with a 1 × 1 FOV in a single shot. This novel advance enables the extraction of photoreceptor-based biomarkers with ease and spatiotemporal monitoring of individual photoreceptors. We compare our findings with AO-assisted ophthalmoscopes, highlighting the potential of FF-OCT, as a compact system, to become a routine clinical imaging technique.
在生物学和医学领域，将高分辨率与大视野（FOV）相结合至关重要，但在对诸如人类视网膜等非平面活体样本进行成像时，这尤其具有挑战性。实际上，高分辨率通常是通过自适应光学（AO）和扫描方法实现的，这会大幅减小有用视野并增加系统复杂性。另一种技术是时域全场光学相干断层扫描（FF - OCT），它已显示出在高分辨率视网膜成像方面的潜力。在此，我们为FF - OCT引入相干门整形，以光学方式塑造相干门几何形状以匹配样本曲率，从而实现比以往更大的视野。使用该仪器，我们在无AO且单次成像视野为1×1的情况下，获得了靠近中央凹中心的活体人类光感受器的高分辨率图像。这一新颖进展使得能够轻松提取基于光感受器的生物标志物，并对单个光感受器进行时空监测。我们将我们的研究结果与AO辅助检眼镜进行比较，突出了FF - OCT作为一种紧凑型系统成为常规临床成像技术的潜力。
