School and Hospital of Stomatology, Fujian Medical University, Fuzhou, Fujian 350000, China.
Stomatological Key Laboratory of Fujian College and University, Fujian Medical University, Fuzhou, 350002 Fujian, China.
Biomed Res Int. 2020 Sep 19;2020:4567049. doi: 10.1155/2020/4567049. eCollection 2020.
Treatments for infectious bone defects such as periodontitis require antibacterial and osteogenic differentiation capabilities. Nanotechnology has prompted the development of multifunctional material. In this research, we aim to synthesize a nanoparticle that can eliminate periodontal pathogenic microorganisms and simultaneously stimulate new bone tissue regeneration and mineralization. QAS-modified core-shell mesoporous silica containing Ag nanoparticles (Ag@QHMS) was successfully synthesized through the classic hydrothermal method and surface quaternary ammonium salt functionalization. The Ag@QHMS in vitro antibacterial activity was explored via coculture with , , and biofilms. Bone mesenchymal stem cells (BMSCs) were selected for observing cytotoxicity, apoptosis, and osteogenic differentiation. Ag@QHMS showed a good sustained release profile of Ag and a QAS-grafted mesoporous structure. Compared with the single-contact antibacterial activity of QHMS, Ag@QHMS exhibited a more efficient and stable concentration-dependent antimicrobial efficacy; the minimum inhibitory concentration was within 100 g/ml, which was below the BMSC biocompatibility concentration (200 g/ml). Thus, apoptosis would not occur while promoting the increased expression of osteogenic-associated factors, such as runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), osteopontin (OPN), osteocalcin (OCN), bone sialoprotein (BSP), and collagen type 1 (COL-1). A safe concentration of particles can stimulate cell alkaline phosphatase and matrix calcium salt deposition. The dual antibacterial effect from the direct contact killing of QAS and the sustained release of Ag nanoparticles, along with the Ag-promoted osteogenic differentiation, had been verified and utilized in Ag@QHMS. This system demonstrates the potential for utilizing pluripotent biomaterials to treat complex lesions.
针对牙周炎等感染性骨缺损的治疗需要具备抗菌和促成骨分化的能力。纳米技术推动了多功能材料的发展。本研究旨在合成一种既能消除牙周致病菌，又能同时刺激新骨组织再生和矿化的纳米颗粒。通过经典的水热法和表面季铵盐功能化成功合成了载银纳米的 QAS 修饰核壳介孔硅（Ag@QHMS）纳米粒子。通过与 、 、 生物膜共培养，研究了 Ag@QHMS 的体外抗菌活性。选择骨髓间充质干细胞（BMSCs）观察细胞毒性、细胞凋亡和成骨分化。Ag@QHMS 表现出良好的 Ag 持续释放特性和 QAS 接枝介孔结构。与 QHMS 的单一接触抗菌活性相比，Ag@QHMS 表现出更高效、更稳定的浓度依赖性抗菌效果；最低抑菌浓度在 100μg/ml 以内，低于 BMSC 生物相容性浓度（200μg/ml）。因此，在促进成骨相关因子（如 runt 相关转录因子 2（RUNX2）、碱性磷酸酶（ALP）、骨桥蛋白（OPN）、骨钙素（OCN）、骨涎蛋白（BSP）和胶原 I 型（COL-1））表达增加的同时，不会发生细胞凋亡。安全浓度的颗粒可以刺激细胞碱性磷酸酶和基质钙盐沉积。通过 QAS 的直接接触杀菌和载银纳米粒子的持续释放产生的双重抗菌作用，以及 Ag 促进的成骨分化，已在 Ag@QHMS 中得到验证和利用。该系统展示了利用多能生物材料治疗复杂病变的潜力。
