Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Institute of Ocean and Earth Sciences, University of Malaya, 50603, Kuala Lumpur, Malaysia.
J Microbiol. 2020 Dec;58(12):988-997. doi: 10.1007/s12275-020-0325-8. Epub 2020 Oct 23.
In this study, bacterial strains Ha5, Ta1, and Jb2 were isolated from different colonies of weaver ant Oecophylla smaragdina. They were identified as bacterial symbionts of the ant belonging to family Acetobacteraceae and were distinguished as different strains based on distinctive random-amplified polymorphic DNA (RAPD) fingerprints. Cells of these bacterial strains were Gram-negative, rod-shaped, aerobic, non-motile, catalase-positive and oxidase-negative. They were able to grow at 15-37°C (optimum, 28-30°C) and in the presence of 0-1.5% (w/v) NaCl (optimum 0%). Their predominant cellular fatty acids were Cω7c, C, Cω8c cyclo, C, and C 2-OH. Strains Ha5, Ta1, and Jb2 shared highest 16S rRNA gene sequence similarity (94.56-94.63%) with Neokomagataea tanensis NBRC106556 of family Acetobacteraceae. Both 16S rRNA gene sequence-based phylogenetic analysis and core gene-based phylogenomic analysis placed them in a distinct lineage in family Acetobacteraceae. These bacterial strains shared higher than species level thresholds in multiple overall genome-relatedness indices which indicated that they belonged to the same species. In addition, they did not belong to any of the current taxa of Acetobacteraceae as they had low pairwise average nucleotide identity (< 71%), in silico DNA-DNA hybridization (< 38%) and average amino acid identity (< 67%) values with all the type members of the family. Based on these results, bacterial strains Ha5, Ta1, and Jb2 represent a novel species of a novel genus in family Acetobacteaceae, for which we propose the name Oecophyllibacter saccharovorans gen. nov. sp. nov., and strain Ha5 as the type strain.
在这项研究中，从不同的绿巨蚁 Oecophylla smaragdina 蚁群中分离出细菌株 Ha5、Ta1 和 Jb2。它们被鉴定为蚂蚁的细菌共生体，属于醋酸杆菌科，根据独特的随机扩增多态性 DNA（RAPD）指纹图谱，被区分成不同的菌株。这些细菌菌株的细胞呈革兰氏阴性、杆状、需氧、非运动性、过氧化氢酶阳性和氧化酶阴性。它们能够在 15-37°C（最佳温度 28-30°C）和 0-1.5%（w/v）NaCl（最佳 0%）的存在下生长。它们主要的细胞脂肪酸为 Cω7c、C、Cω8c 环、C 和 C 2-OH。菌株 Ha5、Ta1 和 Jb2 与醋酸杆菌科的 Neokomagataea tanensis NBRC106556 的 16S rRNA 基因序列相似性最高（94.56-94.63%）。基于 16S rRNA 基因序列的系统发育分析和基于核心基因的系统基因组分析将它们置于醋酸杆菌科的一个独特谱系中。这些细菌菌株在多个全基因组相关指数中具有高于种水平的阈值，这表明它们属于同一物种。此外，它们不属于醋酸杆菌科的任何现有分类群，因为它们与该科的所有模式成员的成对平均核苷酸同一性（<71%）、体外 DNA-DNA 杂交（<38%）和平均氨基酸同一性（<67%）值都较低。基于这些结果，细菌株 Ha5、Ta1 和 Jb2 代表醋酸杆菌科中的一个新属的新种，我们提议将其命名为 Oecophyllibacter saccharovorans gen. nov. sp. nov.，并将菌株 Ha5 作为模式株。
