Division of Polar Life Sciences, Korea Polar Research Institute, 26 Songdomirae-ro, Yeonsu-gu, Incheon 21990, Republic of Korea.
School of Earth and Environmental Sciences, Seoul National University, Seoul 08826, Republic of Korea.
Int J Syst Evol Microbiol. 2020 Nov;70(11):5918-5925. doi: 10.1099/ijsem.0.004495. Epub 2020 Oct 9.
Two Gram-stain-negative, facultative anaerobic, chemoheterotrophic, pink-coloured, rod-shaped and non-motile bacterial strains, PAMC 26568 and PAMC 26569, were isolated from an Antarctic lichen. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains PAMC 26568 and PAMC 26569 belong to the family and the most closely related species are (96.1 %), (95.9 %) and (95.7 %). Phylogenomic and genomic relatedness analyses showed that strains PAMC 26568 and PAMC 26569 are clearly distinguished from other genera in the family by average nucleotide identity values (<72.8 %) and the genome-to-genome distance values (<22.5 %). Genomic analysis revealed that strains PAMC 26568 and PAMC 26569 do not contain genes involved in atmospheric nitrogen fixation and utilization of sole carbon compounds such as methane and methanol. Instead, strains PAMC 26568 and PAMC 26569 possess genes to utilize nitrate and nitrite and certain monosaccharides and disaccharides. The major fatty acids (>10 %) are summed feature 8 (C ω7 and/or C ω6; 40.3-40.4 %), C 2OH (22.7-23.7 %) and summed feature 2 (C 3OH and/or C iso I; 12.0 % in PAMC 26568). The major respiratory quinone is Q-10. The genomic DNA G+C content of PAMC 26568 and PAMC 26569 is 64.6 %. Their distinct phylogenetic position and some physiological characteristics distinguish strains PAMC 26568 and PAMC 26569 from other genera in the family supporting the proposal of gen. nov., with the type species sp. nov. (type strain, PAMC 26569=KCCM 43315=JCM 33604).
两株革兰氏阴性、兼性厌氧、化能异养、粉红色、杆状、非运动性细菌菌株 PAMC 26568 和 PAMC 26569 从南极地衣中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，菌株 PAMC 26568 和 PAMC 26569 属于 科，与最密切相关的物种是 （96.1%）、 （95.9%）和 （95.7%）。系统基因组和基因组相关性分析表明，菌株 PAMC 26568 和 PAMC 26569 通过平均核苷酸同一性值（<72.8%）和基因组到基因组距离值（<22.5%）与 科中的其他属明显区分开来。基因组分析表明，菌株 PAMC 26568 和 PAMC 26569 不包含参与大气固氮和利用甲烷和甲醇等单一碳化合物的基因。相反，菌株 PAMC 26568 和 PAMC 26569 具有利用硝酸盐和亚硝酸盐以及某些单糖和二糖的基因。主要脂肪酸（>10%）是总和特征 8（Cω7 和/或 Cω6；40.3-40.4%）、C 2OH（22.7-23.7%）和总和特征 2（C 3OH 和/或 C iso I；在 PAMC 26568 中为 12.0%）。主要呼吸醌是 Q-10。PAMC 26568 和 PAMC 26569 的基因组 DNA G+C 含量为 64.6%。菌株 PAMC 26568 和 PAMC 26569 的独特系统发育位置和一些生理特征将它们与 科中的其他属区分开来，支持了 属的新提议，其模式种为 种. nov.（模式株，PAMC 26569=KCCM 43315=JCM 33604）。
