S.N. Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, Russia.
Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, Russia.
Int J Syst Evol Microbiol. 2020 Aug;70(8):4591-4601. doi: 10.1099/ijsem.0.004318. Epub 2020 Jul 13.
Gram-negative, aerobic, chemo-organotrophic and bacteriochlorophyll -containing bacterial strains, KEBCLARHB70R, KAMCLST3051 and KAMCLST3152, were isolated from the thalli of and lichens. Cells from the strains were coccoid and reproduced by binary division. They were motile at the early stages of growth and utilized sugars and alcohols. All strains were psychrophilic and acidophilic, capable of growth between pH 3.5 and 7.5 (optimum, pH 5.5), and at 4-30 °C (optimum, 10-15 °C). The major fatty acids were C ω7 and C; the lipids were phosphatidylcholines, phosphatidylethanolamines, phosphatidic acids, phosphatidylglycerol, glycolipids, diphosphatidylglycerol and polar lipids with an unknown structure. The quinone was Q-10. The DNA G+C content was 67.8 mol%. Comparative 16S rRNA gene analysis together with other data, supported that the strains, KEBCLARHB70R, KAMCLST3051 and KAMCLST3152 belonged to the same species. Whole genome analysis of the strain KEBCLARHB70R and average amino acid identity values confirmed its distinctive phylogenetic position within the family . Phenotypic, ecological and genomic characteristics distinguished strains KEBCLARHB70R, KAMCLST3051 and KAMCLST3152 from all genera in the family . Therefore, we propose a novel genus and a novel species, gen. nov., sp. nov., for these novel members. Strain KEBCLARHB70R (=KCTC 72321=VKM B-3305) has been designated as the type strain.
从 和 地衣的藻丝体中分离到革兰氏阴性、需氧、化能有机营养和含有细菌叶绿素的细菌菌株 KEBCLARHB70R、KAMCLST3051 和 KAMCLST3152。这些菌株的细胞呈球菌状，通过二分分裂繁殖。它们在生长的早期阶段是能动的，能够利用糖和醇。所有菌株均为嗜冷嗜酸，能够在 pH 值 3.5 到 7.5 之间（最佳 pH 值为 5.5）和 4-30°C（最佳温度为 10-15°C）生长。主要脂肪酸为 C ω7 和 C ；脂类为磷脂酰胆碱、磷脂酰乙醇胺、磷脂酸、磷脂甘油、糖脂、双磷脂酰甘油和具有未知结构的极性脂类。醌类为 Q-10。DNA G+C 含量为 67.8 mol%。16S rRNA 基因分析和其他数据的比较支持菌株 KEBCLARHB70R、KAMCLST3051 和 KAMCLST3152 属于同一物种。KEBCLARHB70R 菌株的全基因组分析和平均氨基酸同一性值证实了其在 科内的独特进化地位。表型、生态和基因组特征将菌株 KEBCLARHB70R、KAMCLST3051 和 KAMCLST3152 与该科中的所有属区分开来。因此，我们提出了一个新属和一个新种，即 属，新种，用于这些新的 成员。KEBCLARHB70R 菌株（=KCTC 72321=VKM B-3305）已被指定为模式菌株。
