Research and Development Department, Chemyunion Química Ltda, Sorocaba, Brazil.
Laboratory of Phytomedicines, Pharmacology and Biotechnology (PhytoPharmaTech), Department of Biophysics and Pharmacology, Institute of Biosciences, São Paulo State University (Unesp), Botucatu, Brazil.
J Cosmet Dermatol. 2021 Jul;20(7):2190-2202. doi: 10.1111/jocd.13818. Epub 2020 Nov 10.
The use of the injectable products for soft tissue augmentation and treatment of skin aging is an uncomfortable, invasive and related to several complications, and chronic reactions, mainly after long-term application. Efforts to develop new topically active anti-aging products with fewer adverse effects are a huge challenge that should be faced.
We evaluated the anti-aging effects of a phytocosmetic preparation containing Thymus vulgaris associated with lecithin (ThymLec) on the facial wrinkles, expression lines, and face oval remodeling using a double-blind placebo-controlled clinical trial and in vitro cell culture assays.
A clinical trial was conducted to evaluate the effects of ThymLec 2% on the area, length, and depth of the perioral and crow's feet wrinkles, nasolabial and smile lines, as well as face oval remodeling in female volunteers using a sophisticated Bio3D Structured-light Scanner. In the in vitro studies using 3T3-L1 mouse embryonic fibroblasts, adiponectin was measured by immunoenzymatic assay, adipogenesis by the AdipoRed reagent method, and the PPAR-γ expression by RT-PCR analysis.
Topical treatment with ThymLec 2% reduced facial wrinkles and expression lines promoting a face oval remodeling. In the in vitro studies, ThymLec upregulated the PPAR-γ expression increasing adiponectin production and stimulating the adipogenesis process.
The phytocosmetic preparation containing Thymus vulgaris and lecithin is an innovative and safe topical anti-aging product promoting fat tissue augmentation by adipogenesis stimulation via the upregulation of PPAR-γ expression and adiponectin production.
软组织填充和皮肤老化治疗用的注射产品存在不舒适、有创性，并且与多种并发症和慢性反应相关，主要出现在长期应用后。开发具有较少不良反应的新型局部活性抗老化产品是一个巨大的挑战，应该得到应对。
我们评估了一种含有百里香（Thymus vulgaris）和卵磷脂的植物化妆品制剂（ThymLec）对面部皱纹、表情线和面部重塑的抗衰老作用，采用了双盲安慰剂对照临床试验和体外细胞培养试验。
我们进行了一项临床试验，使用一种复杂的 Bio3D Structured-light Scanner 评估了 2%ThymLec 对面部皱纹（口周和鱼尾纹）、鼻唇沟和微笑线的面积、长度和深度，以及女性志愿者面部重塑的影响。在使用 3T3-L1 小鼠胚胎成纤维细胞的体外研究中，通过免疫酶测定法测量脂联素，使用 AdipoRed 试剂法测量脂肪生成，通过 RT-PCR 分析测量 PPAR-γ 表达。
局部应用 2%ThymLec 可减少面部皱纹和表情线，促进面部重塑。在体外研究中，ThymLec 上调了 PPAR-γ 的表达，增加了脂联素的产生，并刺激了脂肪生成过程。
含有百里香和卵磷脂的植物化妆品制剂是一种创新且安全的局部抗老化产品，通过上调 PPAR-γ 的表达和增加脂联素的产生来刺激脂肪组织生成，从而促进脂肪组织的增加。
