Andreatta Francesco, Beccaceci Giulia, Fortuna Nicolò, Celotti Martina, De Felice Dario, Lorenzoni Marco, Foletto Veronica, Genovesi Sacha, Rubert Josep, Alaimo Alessandro
Department of Cellular, Computational and Integrative Biology (CIBIO), University of Trento, Via Sommarive 9, 38123 Trento, Italy.
Interdisciplinary Research Structure of Biotechnology and Biomedicine, Department of Biochemistry and Molecular Biology, Universitat de Valencia, 46100 Burjassot, Spain.
Cancers (Basel). 2020 Nov 9;12(11):3303. doi: 10.3390/cancers12113303.
Glioblastoma (GB) is the most frequent and aggressive type of glioma. The lack of reliable GB models, together with its considerable clinical heterogeneity, has impaired a comprehensive investigation of the mechanisms that lead to tumorigenesis, cancer progression, and response to treatments. Recently, 3D cultures have opened the possibility to overcome these challenges and cerebral organoids are emerging as a leading-edge tool in GB research. The opportunity to easily engineer brain organoids via gene editing and to perform co-cultures with patient-derived tumor spheroids has enabled the analysis of cancer development in a context that better mimics brain tissue architecture. Moreover, the establishment of biobanks from GB patient-derived organoids represents a crucial starting point to improve precision medicine therapies. This review exemplifies relevant aspects of 3D models of glioblastoma, with a specific focus on organoids and their involvement in basic and translational research.
胶质母细胞瘤（GB）是最常见且侵袭性最强的胶质瘤类型。缺乏可靠的GB模型，再加上其显著的临床异质性，阻碍了对导致肿瘤发生、癌症进展及治疗反应的机制进行全面研究。最近，三维培养为克服这些挑战带来了可能，脑类器官正成为GB研究中的前沿工具。通过基因编辑轻松构建脑类器官并与患者来源的肿瘤球体进行共培养，使得在更能模拟脑组织架构的背景下分析癌症发展成为可能。此外，建立来自GB患者来源类器官的生物样本库是改善精准医学治疗的关键起点。本综述举例说明了胶质母细胞瘤三维模型的相关方面，特别关注类器官及其在基础研究和转化研究中的作用。
