Diez-Escudero Anna, Harlin Hugo, Isaksson Per, Persson Cecilia
Division of Applied Materials Science, Department of Materials Science and Engineering, Uppsala University, Uppsala, Sweden.
Division of Applied Mechanics, Department of Materials Science and Engineering, Uppsala University, Uppsala, Sweden.
J Tissue Eng. 2020 Nov 6;11:2041731420956541. doi: 10.1177/2041731420956541. eCollection 2020 Jan-Dec.
Three different triply periodic minimal surfaces (TPMS) with three levels of porosity within those of cancellous bone were investigated as potential bone scaffolds. TPMS have emerged as potential designs to resemble the complex mechanical and mass transport properties of bone. Diamond, Schwarz, and Gyroid structures were 3D printed in polylactic acid, a resorbable medical grade material. The 3D printed structures were investigated for printing feasibility, and assessed by morphometric studies. Mechanical properties and permeability investigations resulted in similar values to cancellous bone. The morphometric analyses showed three different patterns of pore distribution: mono-, bi-, and multimodal pores. Subsequently, biological activity investigated with pre-osteoblastic cell lines showed no signs of cytotoxicity, and the scaffolds supported cell proliferation up to 3 weeks. Cell differentiation investigated by alkaline phosphatase showed an improvement for higher porosities and multimodal pore distributions, suggesting a higher dependency on pore distribution and size than the level of interconnectivity.
研究了三种不同的三重周期极小曲面（TPMS），其孔隙率处于松质骨的三个水平，作为潜在的骨支架。TPMS已成为类似骨复杂力学和传质特性的潜在设计。菱形、施瓦茨和类螺旋结构用聚乳酸（一种可吸收的医用级材料）进行3D打印。对3D打印结构进行了打印可行性研究，并通过形态计量学研究进行评估。力学性能和渗透性研究得出了与松质骨相似的值。形态计量分析显示出三种不同的孔隙分布模式：单峰、双峰和多峰孔隙。随后，用前成骨细胞系研究生物活性，未显示细胞毒性迹象，并且支架在长达3周的时间内支持细胞增殖。通过碱性磷酸酶研究细胞分化表明，对于较高孔隙率和多峰孔隙分布有改善，这表明对孔隙分布和大小的依赖性高于互连水平。
