Yang Dejun, Qiu Qiong, Xu Linhong, Xu Yumei, Wang Yi
Institute of Tropical Forestry, Yunnan Academy of Forestry, Puwen, People's Republic of China.
Laboratory of Forest Plant Cultivation and Utilization, Yunnan Academy of Forestry, Kunming, People's Republic of China.
Mitochondrial DNA B Resour. 2019 Dec 12;5(1):220-221. doi: 10.1080/23802359.2019.1699470.
The first complete chloroplast genome (cpDNA) sequence of was determined from Illumina HiSeq pair-end sequencing data in this study. The cpDNA is 157,688 bp in length, contains a large single copy region (LSC) of 85,994 bp and a small single copy region (SSC) of 17,656 bp, which were separated by a pair of inverted repeats (IR) regions of 27,017 bp. The genome contains 130 genes, including 85 protein-coding genes, 8 ribosomal RNA genes, and 37 transfer RNA genes. The overall GC content of the whole genome is 36.2%, and the corresponding values of the LSC, SSC, and IR regions are 33.6%, 30.2%, and 42.2%, respectively. Further phylogenomic analysis showed that and clustered in a clade in order Malpighiales.
本研究通过Illumina HiSeq双端测序数据确定了首个完整的叶绿体基因组（cpDNA）序列。该cpDNA长度为157,688 bp，包含一个85,994 bp的大单拷贝区域（LSC）和一个17,656 bp的小单拷贝区域（SSC），它们被一对27,017 bp的反向重复（IR）区域隔开。该基因组包含130个基因，包括85个蛋白质编码基因、8个核糖体RNA基因和37个转运RNA基因。全基因组的总体GC含量为36.2%，LSC、SSC和IR区域的相应值分别为33.6%、30.2%和42.2%。进一步的系统发育基因组分析表明，[物种名1]和[物种名2]在金虎尾目一个分支中聚类。
