Luo Ting, Li Yunqing, Yuan Xiaolong, Wang Yi
Laboratory of Forest Plant Cultivation and Utilization, Yunnan Academy of Forestry, Kunming, China.
Mitochondrial DNA B Resour. 2019 Nov 12;4(2):3977-3978. doi: 10.1080/23802359.2019.1688720.
The first complete chloroplast genome (cpDNA) sequence of was determined from Illumina HiSeq pair-end sequencing data in this study. The cpDNA is 150,349 bp in length, contains a large single-copy region (LSC) of 100,582 bp and a small single-copy region (SSC) of 19,455 bp, which were separated by a pair of inverted repeat (IR) regions of 15,156 bp. The genome contains 120 genes, including 79 protein-coding genes, 8 ribosomal RNA genes, and 33 transfer RNA genes. The overall GC content of the whole genome is 34.0%, and the corresponding values of the LSC, SSC, and IR regions are 31.6%, 30.4%, and 44.2%, respectively. Further phylogenomic analysis showed that and clustered in a clade in family Lycopodiaceae.
本研究通过Illumina HiSeq双端测序数据确定了首个完整的叶绿体基因组（cpDNA）序列。该cpDNA长度为150,349 bp，包含一个100,582 bp的大单拷贝区域（LSC）和一个19,455 bp的小单拷贝区域（SSC），它们被一对15,156 bp的反向重复（IR）区域隔开。该基因组包含120个基因，包括79个蛋白质编码基因、8个核糖体RNA基因和33个转运RNA基因。全基因组的总体GC含量为34.0%，LSC、SSC和IR区域的相应值分别为31.6%、30.4%和44.2%。进一步的系统发育基因组分析表明，[物种名称]和[物种名称]在石松科的一个分支中聚类。
