Zhang Jinfeng, Li Yunqing, Wang Yi
Laboratory of Forest Plant Cultivation and Utilization, Yunnan Academy of Forestry, Kunming, People's Republic of China.
Mitochondrial DNA B Resour. 2020 Jan 20;5(1):711-712. doi: 10.1080/23802359.2020.1714504.
The first complete chloroplast genome (cpDNA) sequence of was determined from Illumina HiSeq pair-end sequencing data in this study. The cpDNA is 154,416 bp in length, contains a large single copy region (LSC) of 84,405 bp and a small single copy region (SSC) of 18,285 bp, which were separated by a pair of inverted repeats (IR) regions of 25,863 bp. The genome contains 130 genes, including 85 protein-coding genes, 8 ribosomal RNA genes, and 37 transfer RNA genes. The overall GC content of the whole genome is 38.0%, and the corresponding values of the LSC, SSC, and IR regions are 36.0%, 31.8%, and 43.4%, respectively. Further phylogenomic analysis showed that in a unique clade in Gentianaceae family.
本研究通过Illumina HiSeq双端测序数据确定了首个完整的叶绿体基因组(cpDNA)序列。该cpDNA长度为154,416 bp,包含一个84,405 bp的大单拷贝区域(LSC)和一个18,285 bp的小单拷贝区域(SSC),它们被一对25,863 bp的反向重复(IR)区域隔开。该基因组包含130个基因,其中包括85个蛋白质编码基因、8个核糖体RNA基因和37个转移RNA基因。全基因组的总体GC含量为38.0%,LSC、SSC和IR区域的相应值分别为36.0%、31.8%和43.4%。进一步的系统发育基因组分析表明,其在龙胆科的一个独特分支中。