Zhang Jinfeng, Li Yunqing, Yuan Xiaolong, Wang Yi
Laboratory of Forest Plant Cultivation and Utilization, Yunnan Academy of Forestry, Kunming, People's Republic of China.
Mitochondrial DNA B Resour. 2020 Jan 20;5(1):718-719. doi: 10.1080/23802359.2020.1714509.
The first complete chloroplast genome (cpDNA) sequence of was determined from Illumina HiSeq pair-end sequencing data in this study. The cpDNA is 154,734 bp in length, contains a large single copy region (LSC) of 88,418 bp and a small single copy region (SSC) of 18,790 bp, which were separated by a pair of inverted repeats (IR) regions of 23,763 bp. The genome contains 127 genes, including 84 protein-coding genes, 8 ribosomal RNA genes, and 35 transfer RNA genes. The overall GC content of the whole genome is 36.3%, and the corresponding values of the LSC, SSC, and IR regions are 33.8%, 30.2%, and 43.3%, respectively. Further phylogenomic analysis showed that in Pterocarpus genus clade in Papilionoideae subfamily.
本研究通过Illumina HiSeq双末端测序数据确定了首个完整的叶绿体基因组(cpDNA)序列。该cpDNA长度为154,734 bp,包含一个88,418 bp的大单拷贝区域(LSC)和一个18,790 bp的小单拷贝区域(SSC),它们被一对23,763 bp的反向重复(IR)区域隔开。该基因组包含127个基因,包括84个蛋白质编码基因、8个核糖体RNA基因和35个转移RNA基因。全基因组的总体GC含量为36.3%,LSC、SSC和IR区域的相应值分别为33.8%、30.2%和43.3%。进一步的系统发育基因组分析表明,其处于蝶形花亚科紫檀属分支中。