• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

黄芪不同分子量多糖的结构表征及免疫活性筛选

Structural Characterization and Immune Activity Screening of Polysaccharides With Different Molecular Weights From Astragali Radix.

作者信息

Li Ke, Cao Y-X, Jiao S-M, Du G-H, Du Y-G, Qin X-M

机构信息

Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan, China.

Institute of Process Engineering, Chinese Academy of Sciences, Beijing, China.

出版信息

Front Pharmacol. 2020 Nov 24;11:582091. doi: 10.3389/fphar.2020.582091. eCollection 2020.

DOI:10.3389/fphar.2020.582091
PMID:33390949
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7774520/
Abstract

Saccharides are the most abundant substance with the strongest immunological activity in Astragali Radix (AR). However, systematic structure study and immunoactivity screening of polysaccharides with different molecular weights (Mw) in AR have yet to be conducted. In this study, Astragalus polysaccharides (APSs) were divided into three fragments of different Mw values, >2,000 kDa (APS-Ⅰ), about 10 kDa (APS-Ⅱ), and about 300 Da (APS-Ⅲ), by using ultrafiltration for the first time. The structural differences of the three products were determined on the basis of monosaccharide composition, FT-IR spectrum, linkage analysis, and nuclear magnetic resonance analysis. Cellular immune activity experiments and cyclophosphamide immunosuppression animal model experiments for nonspecific and specific immunoactivity screening were applied to identify the most immunogenic fragment in APSs. Linkage analysis results showed that APS-Ⅰ, APS-Ⅱ, and APS-Ⅲ have different attachment sites of monosaccharide residues. Immune screening experiments indicated that the Mw of the APSs influenced their activity, and APS-Ⅱ had the strongest immunoenhancing activity among the products. This research may serve as a reference for further study on APSs with different structures and immune activities, and as a guidance for the quality control of APSs and the development of new APS products.

摘要

糖类是黄芪中含量最为丰富且免疫活性最强的物质。然而，尚未对黄芪中不同分子量（Mw）的多糖进行系统的结构研究和免疫活性筛选。在本研究中，首次采用超滤法将黄芪多糖（APSs）分为分子量不同的三个片段，即>2000 kDa（APS-Ⅰ）、约10 kDa（APS-Ⅱ）和约300 Da（APS-Ⅲ）。基于单糖组成、傅里叶变换红外光谱（FT-IR）、连接分析和核磁共振分析确定了这三种产物的结构差异。应用细胞免疫活性实验和环磷酰胺免疫抑制动物模型实验进行非特异性和特异性免疫活性筛选，以鉴定APSs中免疫原性最强的片段。连接分析结果表明，APS-Ⅰ、APS-Ⅱ和APS-Ⅲ的单糖残基连接位点不同。免疫筛选实验表明，APSs的分子量影响其活性，且APS-Ⅱ在这些产物中具有最强的免疫增强活性。本研究可为进一步研究具有不同结构和免疫活性的APSs提供参考，并为APSs的质量控制和新型APS产品的开发提供指导。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/7774520/4c44ec8bbdaf/fphar-11-582091-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/7774520/04eed65dd668/fphar-11-582091-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/7774520/af56d2fd0300/fphar-11-582091-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/7774520/54b81239d0ec/fphar-11-582091-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/7774520/5d97f4329219/fphar-11-582091-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/7774520/b9346fa2a016/fphar-11-582091-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/7774520/93ecf2ee068a/fphar-11-582091-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/7774520/06b84d44c4bf/fphar-11-582091-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/7774520/6c06e364dcf4/fphar-11-582091-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/7774520/4ff152ead706/fphar-11-582091-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/7774520/4b6876fa9eb2/fphar-11-582091-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/7774520/4c44ec8bbdaf/fphar-11-582091-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/7774520/04eed65dd668/fphar-11-582091-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/7774520/af56d2fd0300/fphar-11-582091-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/7774520/54b81239d0ec/fphar-11-582091-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/7774520/5d97f4329219/fphar-11-582091-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/7774520/b9346fa2a016/fphar-11-582091-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/7774520/93ecf2ee068a/fphar-11-582091-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/7774520/06b84d44c4bf/fphar-11-582091-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/7774520/6c06e364dcf4/fphar-11-582091-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/7774520/4ff152ead706/fphar-11-582091-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/7774520/4b6876fa9eb2/fphar-11-582091-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8af4/7774520/4c44ec8bbdaf/fphar-11-582091-g010.jpg

相似文献

1
Structural Characterization and Immune Activity Screening of Polysaccharides With Different Molecular Weights From Astragali Radix.黄芪不同分子量多糖的结构表征及免疫活性筛选
Front Pharmacol. 2020 Nov 24;11:582091. doi: 10.3389/fphar.2020.582091. eCollection 2020.
2
Structural Characterization and Screening for Anti-inflammatory Activity of Polysaccharides with Different Molecular Weights from Astragali Radix.黄芪中不同分子量多糖的结构特征分析及抗炎活性筛选。
Chem Biodivers. 2024 Jul;21(7):e202400262. doi: 10.1002/cbdv.202400262. Epub 2024 Jun 7.
3
Relationship Between the Structure and Immune Activity of Components From the Active Polysaccharides APS-II of Astragali Radix by Enzymolysis of Endo α-1,4-Glucanase.通过内切α-1,4-葡聚糖酶酶解黄芪活性多糖APS-II的组分结构与免疫活性之间的关系
Front Pharmacol. 2022 Feb 25;13:839635. doi: 10.3389/fphar.2022.839635. eCollection 2022.
4
Screening and structure study of active components of Astragalus polysaccharide for injection based on different molecular weights.基于不同分子量的黄芪多糖注射液活性成分的筛选与结构研究。
J Chromatogr B Analyt Technol Biomed Life Sci. 2020 Sep 1;1152:122255. doi: 10.1016/j.jchromb.2020.122255. Epub 2020 Jun 24.
5
Molecular weight and monosaccharide composition of Astragalus polysaccharides.黄芪多糖的分子量及单糖组成
Molecules. 2008 Oct 1;13(10):2408-15. doi: 10.3390/molecules13102408.
6
Relationship between molecular weight, monosaccharide composition and immunobiologic activity of Astragalus polysaccharides.黄芪多糖的分子量、单糖组成与免疫生物学活性之间的关系
Glycoconj J. 2016 Oct;33(5):755-61. doi: 10.1007/s10719-016-9669-z. Epub 2016 Apr 30.
7
UHPLC Q-Exactive MS-Based Serum Metabolomics to Explore the Effect Mechanisms of Immunological Activity of Polysaccharides With Different Molecular Weights.基于超高效液相色谱-四极杆-静电场轨道阱质谱的血清代谢组学研究不同分子量多糖免疫活性的作用机制
Front Pharmacol. 2020 Dec 15;11:595692. doi: 10.3389/fphar.2020.595692. eCollection 2020.
8
Structure Differences of Water Soluble Polysaccharides in Induced by Origin and Their Bioactivity.产地诱导的水溶性多糖的结构差异及其生物活性
Foods. 2021 Jul 29;10(8):1755. doi: 10.3390/foods10081755.
9
Repair Effects of Astragalus Polysaccharides with Different Molecular Weights on Oxidatively Damaged HK-2 Cells.不同分子量黄芪多糖对氧化损伤 HK-2 细胞的修复作用。
Sci Rep. 2019 Jul 8;9(1):9871. doi: 10.1038/s41598-019-46264-y.
10
Screening of Polysaccharides with Different Molecular Weights and Evaluation of Their Immunomodulatory Activity In Vitro and In Vivo.不同分子量多糖的筛选及其体内外免疫调节活性评价。
Molecules. 2022 Aug 25;27(17):5454. doi: 10.3390/molecules27175454.

引用本文的文献

1
Effects of Low-Protein Amino Acid-Balanced Diets and Polysaccharides on Production Performance, Antioxidants, Immunity, and Lipid Metabolism in Heat-Stressed Laying Hens.低蛋白氨基酸平衡日粮和多糖对热应激蛋鸡生产性能、抗氧化能力、免疫力及脂质代谢的影响
Animals (Basel). 2025 Aug 14;15(16):2385. doi: 10.3390/ani15162385.
2
Industrial Applications, Principal Sources, and Extraction of Galactomannans: A Review.半乳甘露聚糖的工业应用、主要来源及提取：综述
Foods. 2025 Apr 30;14(9):1587. doi: 10.3390/foods14091587.
3
Post-harvest processing methods have critical roles on the contents of active metabolites and pharmacological effects of .

本文引用的文献

1
[Interaction between homologous functional food Astragali Radix and intestinal flora].[同源功能性食品黄芪与肠道菌群的相互作用]
Zhongguo Zhong Yao Za Zhi. 2020 Jun;45(11):2486-2492. doi: 10.19540/j.cnki.cjcmm.20200119.401.
2
A Review of the Pharmacological Action of Astragalus Polysaccharide.黄芪多糖的药理作用综述
Front Pharmacol. 2020 Mar 24;11:349. doi: 10.3389/fphar.2020.00349. eCollection 2020.
3
Repair Effects of Astragalus Polysaccharides with Different Molecular Weights on Oxidatively Damaged HK-2 Cells.
收获后加工方法对……的活性代谢物含量和药理作用具有关键作用。 （原句中“of”后面缺少具体内容）
Front Pharmacol. 2024 Dec 9;15:1489777. doi: 10.3389/fphar.2024.1489777. eCollection 2024.
4
The Influence and Mechanisms of Natural Plant Polysaccharides on Intestinal Microbiota-Mediated Metabolic Disorders.天然植物多糖对肠道微生物群介导的代谢紊乱的影响及其机制
Foods. 2024 Nov 30;13(23):3882. doi: 10.3390/foods13233882.
5
Astragali radix (Huangqi): a time-honored nourishing herbal medicine.黄芪：一种历史悠久的滋补草药。
Chin Med. 2024 Aug 30;19(1):119. doi: 10.1186/s13020-024-00977-z.
6
Chemical Composition and Therapeutic Effects of Several Astragalus Species (Fabaceae).几种黄芪属植物（豆科）的化学成分和治疗效果。
Dokl Biol Sci. 2024 Oct;518(1):172-186. doi: 10.1134/S0012496624701096. Epub 2024 Aug 11.
7
A Rapid and Accurate UHPLC Method for Determination of Monosaccharides in Polysaccharides of Different Sources of Radix Astragali and Its Immune Activity Analysis.一种快速准确的 UHPLC 法测定不同来源黄芪多糖中单糖组成及其免疫活性分析。
Molecules. 2024 May 13;29(10):2287. doi: 10.3390/molecules29102287.
8
Yogurt Alleviates Cyclophosphamide-Induced Immunosuppression in Mice through D-Lactate.酸奶通过 D-乳酸缓解环磷酰胺诱导的小鼠免疫抑制。
Nutrients. 2024 May 6;16(9):1395. doi: 10.3390/nu16091395.
9
Research Progress of Polysaccharide-Gold Nanocomplexes in Drug Delivery.多糖-金纳米复合物在药物递送中的研究进展
Pharmaceutics. 2024 Jan 9;16(1):88. doi: 10.3390/pharmaceutics16010088.
10
Immunomodulatory Activity and Its Mechanisms of Two Polysaccharides from .两种多糖的免疫调节活性及其机制研究
Molecules. 2023 Dec 20;29(1):50. doi: 10.3390/molecules29010050.
不同分子量黄芪多糖对氧化损伤 HK-2 细胞的修复作用。
Sci Rep. 2019 Jul 8;9(1):9871. doi: 10.1038/s41598-019-46264-y.
4
Effect of Bacillus sp. DU-106 fermentation on Dendrobium officinale polysaccharide: Structure and immunoregulatory activities.芽孢杆菌 DU-106 发酵对铁皮石斛多糖的影响：结构和免疫调节活性。
Int J Biol Macromol. 2019 Aug 15;135:1034-1042. doi: 10.1016/j.ijbiomac.2019.05.203. Epub 2019 Jun 4.
5
Structure Characterization of Honey-Processed Astragalus Polysaccharides and Its Anti-Inflammatory Activity In Vitro.蜂蜜炮制黄芪多糖的结构特征及其体外抗炎活性。
Molecules. 2018 Jan 15;23(1):168. doi: 10.3390/molecules23010168.
6
Extraction of a Novel Cold-Water-Soluble Polysaccharide from Astragalus membranaceus and Its Antitumor and Immunological Activities.从黄芪中提取新型冷水溶性多糖及其抗肿瘤和免疫活性。
Molecules. 2017 Dec 28;23(1):62. doi: 10.3390/molecules23010062.
7
Relationship between molecular weight, monosaccharide composition and immunobiologic activity of Astragalus polysaccharides.黄芪多糖的分子量、单糖组成与免疫生物学活性之间的关系
Glycoconj J. 2016 Oct;33(5):755-61. doi: 10.1007/s10719-016-9669-z. Epub 2016 Apr 30.
8
Effects of the ultra-high pressure on structure and α-glucosidase inhibition of polysaccharide from Astragalus.超高压对黄芪多糖结构及α-葡萄糖苷酶抑制作用的影响
Int J Biol Macromol. 2016 Jun;87:570-6. doi: 10.1016/j.ijbiomac.2016.03.024. Epub 2016 Mar 16.
9
Structural characterization and antioxidant activity in vitro of polysaccharides from angelica and astragalus.当归和黄芪多糖的结构表征及体外抗氧化活性研究。
Carbohydr Polym. 2016 Feb 10;137:154-164. doi: 10.1016/j.carbpol.2015.10.053. Epub 2015 Oct 19.
10
Purification, partial characterization and antioxidant activity of polysaccharides from Glycyrrhiza uralensis.甘草多糖的纯化、部分特性鉴定及抗氧化活性
Int J Biol Macromol. 2015 Aug;79:681-6. doi: 10.1016/j.ijbiomac.2015.05.060. Epub 2015 Jun 5.