Department of Analytical Chemistry, Iuliu Hațieganu University of Medicine and Pharmacy, 4 Louis Pasteur Street, 400349 Cluj-Napoca, Romania.
Department of Pharmaceutical Technology and Biopharmaceutics, Iuliu Hațieganu University of Medicine and Pharmacy, 41 Victor Babes Street, 400012 Cluj-Napoca, Romania.
Biosensors (Basel). 2021 Jan 3;11(1):15. doi: 10.3390/bios11010015.
The aim of this study was to develop a disposable, simple, fast, and sensitive sensor for the simultaneous electrochemical detection of doxorubicin (DOX) and simvastatin (SMV), which could be used in preclinical studies for the development of new pharmaceutical formulations for drug delivery. Firstly, the electrochemical behavior of each molecule was analyzed regarding the influence of electrode material, electrolyte solution, and scan rate. After this, the proper electrode material, electrolyte solution, and scan rate for both active substances were chosen, and a linear sweep voltammetry procedure was optimized for simultaneous detection. Two chronoamperometry procedures were tested, one for the detection of DOX in the presence of SMV, and the other one for the detection of DOX and SMV together. Finally, calibration curves for DOX and SMV in the presence of each other were obtained using both electrochemical methods and the results were compared. The use of amperometry allowed for a better limit of detection (DOX: 0.1 μg/mL; SMV: 0.7 μg/mL) than the one obtained in voltammetry (1.5 μg/mL for both drugs). The limits of quantification using amperometry were 0.5 μg/mL for DOX (dynamic range: 0.5-65 μg/mL) and 2 μg/mL for SMV (dynamic range: 2-65 μg/mL), while using voltammetry 1 μg/mL was obtained for DOX (dynamic range: 1-100 μg/mL) and 5 μg/mL for SMV (dynamic range: 5-100 μg/mL). This detection strategy represents a promising tool for the analysis of new pharmaceutical formulations for targeted drug delivery containing both drugs, whose association was proven to bring benefits in the treatment of cancer.
本研究旨在开发一种用于同时电化学检测阿霉素（DOX）和辛伐他汀（SMV）的一次性、简单、快速和灵敏的传感器，可用于临床前研究，开发用于药物输送的新药物制剂。首先，分析了每个分子的电化学行为，研究了电极材料、电解质溶液和扫描速率对其的影响。然后，选择了两种活性物质的合适电极材料、电解质溶液和扫描速率，并优化了线性扫描伏安法程序以进行同时检测。测试了两种计时安培法程序，一种用于在 SMV 存在下检测 DOX，另一种用于同时检测 DOX 和 SMV。最后，使用两种电化学方法获得了 DOX 和 SMV 彼此存在时的校准曲线，并对结果进行了比较。与伏安法相比，使用安培法可以获得更好的检测限（DOX：0.1μg/mL；SMV：0.7μg/mL）。使用安培法的定量限分别为 DOX 的 0.5μg/mL（动态范围：0.5-65μg/mL）和 SMV 的 2μg/mL（动态范围：2-65μg/mL），而使用伏安法时 DOX 为 1μg/mL（动态范围：1-100μg/mL），SMV 为 5μg/mL（动态范围：5-100μg/mL）。这种检测策略代表了用于分析含有这两种药物的靶向药物输送的新药物制剂的有前途的工具，已证明它们的联合使用在癌症治疗中带来益处。
