Donati Paolo, Pomili Tania, Boselli Luca, Pompa Pier P
Nanobiointeractions and Nanodiagnostics, Istituto Italiano di Tecnologia, Genova, Italy.
Front Bioeng Biotechnol. 2020 Dec 7;8:601216. doi: 10.3389/fbioe.2020.601216. eCollection 2020.
Early diagnostics and point-of-care (POC) devices can save people's lives or drastically improve their quality. In particular, millions of diabetic patients worldwide benefit from POC devices for frequent self-monitoring of blood glucose. Yet, this still involves invasive sampling processes, which are quite discomforting for frequent measurements, or implantable devices dedicated to selected chronic patients, thus precluding large-scale monitoring of the globally increasing diabetic disorders. Here, we report a non-invasive colorimetric sensing platform to identify hyperglycemia from saliva. We designed plasmonic multibranched gold nanostructures, able to rapidly change their shape and color (naked-eye detection) in the presence of hyperglycemic conditions. This "reshaping approach" provides a fast visual response and high sensitivity, overcoming common detection issues related to signal (color intensity) losses and bio-matrix interferences. Notably, optimal performances of the assay were achieved in real biological samples, where the biomolecular environment was found to play a key role. Finally, we developed a dipstick prototype as a rapid home-testing kit.
早期诊断和即时检测（POC）设备可以挽救人们的生命或极大地改善他们的生活质量。特别是，全球数百万糖尿病患者受益于POC设备进行频繁的血糖自我监测。然而，这仍然涉及侵入性采样过程，对于频繁测量来说相当不舒服，或者是专门用于特定慢性病患者的可植入设备，因此排除了对全球范围内日益增加的糖尿病疾病进行大规模监测的可能性。在这里，我们报告了一种用于从唾液中识别高血糖的非侵入性比色传感平台。我们设计了等离子体多分支金纳米结构，在高血糖条件下能够迅速改变其形状和颜色（裸眼检测）。这种“重塑方法”提供了快速的视觉响应和高灵敏度，克服了与信号（颜色强度）损失和生物基质干扰相关的常见检测问题。值得注意的是，该检测方法在实际生物样品中实现了最佳性能，发现生物分子环境在其中起着关键作用。最后，我们开发了一种试纸原型作为快速家庭检测试剂盒。
