Institute for Translational Medicine, the First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, Shenzhen, China.
Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
Exp Dermatol. 2021 Jun;30(6):865-871. doi: 10.1111/exd.14277. Epub 2021 Jan 19.
Chlorogenic acid (CGA) exhibits substantial biological function in antioxidant, antibacterial, anti-lipogenesis and anti-inflammatory activities. Increased sebum production and inflammation are considered important for the development of acne. However, the therapeutic effects of CGA on acne vulgaris remain unexplored. In this study, to assess the effects and underlying mechanisms of CGA on acne, a model of skin inflammation in ears of ICR mouse induced by living Propionibacterium acnes was used. 24 hours after 1.0 × 107 CFU, P. acnes were intradermally injected into the ears of the ICR mouse. 1, 5 and 10 mg of CGA mixed with vaseline were applied to the surface of the skin every 12 hours for 3 days. Then, skin inflammation in the ears was assessed and the change of SREBP1 and TNF-α expression was analysed after CGA treatment. The mechanisms of CGA in anti-inflammatory activity and lipogenesis were also studied in primary sebocytes and HaCaT cells. We found that CGA treatment effectively rescued ear swelling, redness and erythema skin in ears of ICR mouse induced by P. acnes and significantly downregulated the expression of inflammatory cytokines by reducing the activity of the NF-κB signalling pathway. Furthermore, CGA could inhibit lipogenesis at the protein secretion and transcription level by decreasing the AKT/mTOR/SREBP signalling pathway. Our findings suggest that CGA could become a potential alternative drug for the treatment of acne vulgaris.
绿原酸（CGA）在抗氧化、抗菌、抗脂肪生成和抗炎活性方面表现出显著的生物学功能。皮脂分泌增加和炎症被认为是痤疮发展的重要因素。然而，CGA 对寻常痤疮的治疗作用仍未得到探索。在这项研究中，为了评估 CGA 对痤疮的影响及其潜在机制，我们使用了活痤疮丙酸杆菌诱导 ICR 小鼠耳部皮肤炎症的模型。在 1.0×107 CFU 后 24 小时，将 P. acnes 皮内注射到 ICR 小鼠的耳部。将 1、5 和 10mg 的 CGA 与凡士林混合，每 12 小时涂于皮肤表面，连续 3 天。然后评估 CGA 处理后耳部皮肤炎症的变化，并分析 SREBP1 和 TNF-α 表达的变化。还在原代皮脂细胞和 HaCaT 细胞中研究了 CGA 在抗炎活性和脂肪生成中的作用机制。我们发现 CGA 处理可有效挽救由 P. acnes 诱导的 ICR 小鼠耳部肿胀、发红和红斑皮肤，并通过降低 NF-κB 信号通路的活性显著下调炎症细胞因子的表达。此外，CGA 可以通过降低 AKT/mTOR/SREBP 信号通路来抑制蛋白分泌和转录水平的脂肪生成。我们的研究结果表明，CGA 可能成为治疗寻常痤疮的一种潜在替代药物。
