Su Siheng, Wang Jilong, Vargas Evan, Wei Junhua, Martínez-Zaguilán Raul, Sennoune Souad R, Pantoya Michelle L, Wang Shiren, Chaudhuri Jharna, Qiu Jingjing
Department of Mechanical Engineering, Texas Tech University, 2500 Broadway, Lubbock, Texas 79409, United States.
Department of Cell Physiology and Molecular Biophysics, Texas Tech University Health Sciences Center, 3601 Fourth Street, Lubbock, Texas 79430, United States.
ACS Biomater Sci Eng. 2016 Aug 8;2(8):1357-1366. doi: 10.1021/acsbiomaterials.6b00290. Epub 2016 Jul 22.
Brain cancer is a fatal disease that is difficult to treat because of poor targeting and low permeability of chemotherapeutic drugs through the blood brain barrier. In a comparison to current treatments, such as surgery followed by chemotherapy and/or radiotherapy, photothermal therapy is a remarkable noninvasive therapy developed in recent years. In this work, porphyrin immobilized nanographene oxide (PNG) was synthesized and bioconjugated with a peptide to achieve enhanced and targeted photothermal therapy for brain cancer. PNG was dispersed into the agar based artificial tissue model and demonstrated a photo-to-thermal conversion efficiency of 19.93% at a PNG concentration of only 0.5 wt %, with a heating rate of 0.6 °C/s at the beginning of irradiation. In comparison, 0.5 wt % graphene oxide (GO) indicated a photo-to-thermal conversion efficiency of 12.20% and a heating rate of 0.3 °C/s. To actively target brain tumor cells without harming healthy cells and tissues surrounding the laser path, a tripeptide l-arginyl-glycyl-l-aspartic (RGD) was further grafted to PNG. The photothermal therapy effects of PNG-RGD completely eliminated the tumor , indicating its excellent therapeutic effect for the treatment of brain cancer.
脑癌是一种致命疾病，由于化疗药物靶向性差且透过血脑屏障的渗透性低，因而难以治疗。与当前的治疗方法（如手术后进行化疗和/或放疗）相比，光热疗法是近年来开发的一种卓越的非侵入性治疗方法。在这项工作中，合成了卟啉固定化氧化石墨烯（PNG），并将其与一种肽进行生物共轭，以实现增强型和靶向性脑癌光热疗法。将PNG分散到基于琼脂的人工组织模型中，在PNG浓度仅为0.5 wt%时，其光热转换效率为19.93%，在照射开始时加热速率为0.6℃/秒。相比之下，0.5 wt%的氧化石墨烯（GO）的光热转换效率为12.20%，加热速率为0.3℃/秒。为了在不损害激光路径周围健康细胞和组织的情况下主动靶向脑肿瘤细胞，将三肽l-精氨酰-甘氨酰-l-天冬氨酸（RGD）进一步接枝到PNG上。PNG-RGD的光热治疗效果完全消除了肿瘤，表明其对脑癌治疗具有优异的治疗效果。
