Zoidis Evangelos, Pappas Athanasios C, Goliomytis Michael, Simitzis Panagiotis E, Sotirakoglou Kyriaki, Tavrizelou Savvina, Danezis George, Georgiou Constantinos A
Laboratory of Nutritional Physiology and Feeding, Department of Animal Science, Agricultural University of Athens, 11855 Athens, Greece.
Laboratory of Animal Breeding and Husbandry, Department of Animal Science, Agricultural University of Athens, 11855 Athens, Greece.
Antioxidants (Basel). 2021 Jan 9;10(1):80. doi: 10.3390/antiox10010080.
The objective of the present study was to investigate the effect of the natural flavonoid quercetin dietary supplementation on the alteration of egg metallome by applying the basic principles of elemental metabolomics. One hundred and ninety-two laying hens were allocated into 4 treatment groups: the control (C) group that was fed with a commercial basal diet and the other experimental groups that were offered the same diet further supplemented with quercetin at 200, 400 and 800 mg per kg of feed (Q2, Q4 and Q8 group, respectively) for 28 days. The diets contained the same vitamin and mineral premix, thus all birds received the same amount of elements since no differences on feed intake existed. The egg elemental profile consisted of As, Ca, Cd, Co, Cr, Cu, Fe, Mg, Mn, Mo, Ni, Pb, Sb, Se, Sr, V, Zn and was determined using inductively coupled plasma mass spectrometry (ICP-MS). Quercetin supplementation altered the elemental profile. Most notably, quercetin altered the element concentrations predominantly in egg shell and albumen. It increased the concentration of Sb while reduced that of Cr and Se in both egg shell and albumen. Moreover, it increased As, Cd in albumen and V in yolk, while compared to the control, reduced As, Cd, Cr, Cu and V and also raised Ca, Fe, Mg and Ni in egg shell. The presence of quercetin led to differentiation of the deposition of certain trace minerals in egg compartments compared to that of hens fed a basal diet, possibly indicating that tailor made eggs for specific nutritional and health requirements could be created in the future.
本研究的目的是通过应用元素代谢组学的基本原理，研究天然黄酮类化合物槲皮素膳食补充剂对鸡蛋金属组变化的影响。192只蛋鸡被分为4个处理组：对照组（C组）饲喂商业基础日粮，其他实验组饲喂相同日粮，并分别添加200、400和800 mg/kg饲料的槲皮素（分别为Q2、Q4和Q8组），持续28天。日粮中含有相同的维生素和矿物质预混料，由于采食量没有差异，所有鸡摄入的元素量相同。鸡蛋的元素谱包括砷、钙、镉、钴、铬、铜、铁、镁、锰、钼、镍、铅、锑、硒、锶、钒、锌，使用电感耦合等离子体质谱法（ICP-MS）进行测定。补充槲皮素改变了元素谱。最显著的是，槲皮素主要改变了蛋壳和蛋清中的元素浓度。它增加了蛋壳和蛋清中锑的浓度，同时降低了铬和硒的浓度。此外，它增加了蛋清中的砷、镉和蛋黄中的钒，与对照组相比，降低了蛋壳中的砷、镉、铬、铜和钒，同时提高了蛋壳中的钙、铁、镁和镍。与饲喂基础日粮的母鸡相比，槲皮素的存在导致了某些微量矿物质在蛋各部分沉积的差异，这可能表明未来可以生产出满足特定营养和健康需求的特制鸡蛋。
