School of Clinical Dentistry, University of Sheffield, Sheffield, United Kingdom.
Biomed Phys Eng Express. 2020 Feb 18;6(2):025007. doi: 10.1088/2057-1976/ab73f8.
Autologous cancellous-bone grafts are the current gold standard for therapeutic interventions in which bone-regeneration is desired. The main limitations of these implants are the need for a secondary surgical site, creating a wound on the patient, the limited availability of harvest-safe bone, and the lack of structural integrity of the grafts. Synthetic, resorbable, bone-regeneration materials could pose a viable treatment alternative, that could be implemented through 3D-printing. We present here the development of a polylactic acid-hydroxyapatite (PLA-HAp) composite that can be processed through a commercial-grade 3D-printer. We have shown that this material could be a viable option for the development of therapeutic implants for bone regeneration. Biocompatibility in vitro was demonstrated through cell viability studies using the osteoblastic MG63 cell-line, and we have also provided evidence that the presence of HAp in the polymer matrix enhances cell attachment and osteogenicity of the material. We have also provided guidelines for the optimal PLA-HAp ratio for this application, as well as further characterisation of the mechanical and thermal properties of the composite. This study encompasses the base for further research on the possibilities and safety of 3D-printable, polymer-based, resorbable composites for bone regeneration.
自体松质骨移植物是目前治疗骨再生所期望的金标准。这些植入物的主要局限性是需要二次手术部位，即在患者身上造成创伤，可安全采集的骨量有限，以及移植物缺乏结构完整性。可吸收的合成骨再生材料可能是一种可行的治疗替代方案，可以通过 3D 打印来实现。我们在这里介绍了一种聚乳酸-羟基磷灰石（PLA-HAp）复合材料的开发，该材料可通过商业级 3D 打印机进行加工。我们已经证明，这种材料可能是开发用于骨再生的治疗性植入物的可行选择。通过使用成骨细胞 MG63 细胞系进行细胞活力研究证明了体外生物相容性，并且我们还提供了证据表明聚合物基质中 HAp 的存在增强了材料的细胞附着和成骨性。我们还为这种应用提供了 PLA-HAp 最佳比例的指南，以及对复合材料的机械和热性能的进一步表征。这项研究为进一步研究用于骨再生的 3D 可打印聚合物基可吸收复合材料的可能性和安全性奠定了基础。
