Laboratório de Transmissores de Hematozoários, IOC, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, 21040-360, Brazil.
Laboratory for Microbiology, Parasitology and Hygiene (LMPH), University of Antwerp, 2000, Antwerp, Belgium.
Commun Biol. 2021 Jan 15;4(1):67. doi: 10.1038/s42003-020-01601-0.
Deployment of Wolbachia to mitigate dengue (DENV), Zika (ZIKV) and chikungunya (CHIKV) transmission is ongoing in 12 countries. One way to assess the efficacy of Wolbachia releases is to determine invasion rates within the wild population of Aedes aegypti following their release. Herein we evaluated the accuracy, sensitivity and specificity of the Near Infrared Spectroscopy (NIRS) in estimating the time post death, ZIKV-, CHIKV-, and Wolbachia-infection in trapped dead female Ae. aegypti mosquitoes over a period of 7 days. Regardless of the infection type, time post-death of mosquitoes was accurately predicted into four categories (fresh, 1 day old, 2-4 days old and 5-7 days old). Overall accuracies of 93.2, 97 and 90.3% were observed when NIRS was used to detect ZIKV, CHIKV and Wolbachia in dead Ae. aegypti female mosquitoes indicating NIRS could be potentially applied as a rapid and cost-effective arbovirus surveillance tool. However, field data is required to demonstrate the full capacity of NIRS for detecting these infections under field conditions.
在 12 个国家正在部署沃尔巴克氏体以减轻登革热（DENV）、寨卡（ZIKV）和基孔肯雅（CHIKV）的传播。评估沃尔巴克氏体释放效果的一种方法是确定释放后野生埃及伊蚊种群中的入侵率。在此，我们评估了近红外光谱（NIRS）在估计捕获的死亡雌性埃及伊蚊 Aedes aegypti 感染寨卡病毒、基孔肯雅病毒和沃尔巴克氏体后 7 天内死亡时间的准确性、灵敏度和特异性。无论感染类型如何，蚊子死亡后的时间都可以准确地预测为四个类别（新鲜、1 天、2-4 天和 5-7 天）。当 NIRS 用于检测死亡的埃及伊蚊雌性蚊子中的寨卡病毒、基孔肯雅病毒和沃尔巴克氏体时，观察到总体准确率分别为 93.2%、97%和 90.3%，这表明 NIRS 可能被用作一种快速且具有成本效益的虫媒病毒监测工具。然而，需要现场数据来证明 NIRS 在现场条件下检测这些感染的全部能力。
