Meera S, Sarangarajan R, Rajkumar K
Private Practitioner, Sree Sai Dental Care, Chennai, Tamil Nadu, India.
Department of Oral and Maxillofacial Pathology, Madha Dental College, Chennai, Tamil Nadu, India.
J Oral Maxillofac Pathol. 2020 May-Aug;24(2):279-284. doi: 10.4103/jomfp.JOMFP_235_19. Epub 2020 Sep 9.
8-isoprostane is one of the stable oxidative stress marker formed by the lipid peroxidation of arachidonic acid. It is present in detectable quantities in all biological fluids. Elevation of 8-Isoprostane has been reported in various neurological, cardiological disorders, and periodontal diseases.
The present study was conducted to estimate and compare the level of 8-isoprostane in plasma and saliva in patients with oral squamous cell carcinoma (OSCC), oral submucous fibrosis (OSMF), and in controls. The study also aimed to find out if 8-isoprostane can be used as an effective oxidative stress marker in evaluating the disease progression in OSCC.
Plasma and salivary samples were taken from 10 cases each of clinically diagnosed OSMF, clinically and hisotpathologically diagnosed cases of OSCC and controls. The samples were subjected to 8-Isoprostane ELISA procedure and analyzed. Statistical analysis was performed using the SPSS software.
The levels of 8-isoprostane in plasma showed an average increase from normal to OSMF to OSCC but was not statistically significant. The variations in the level of salivary 8-isoprostane were found to be statistically significant ( = 0.037) suggesting that there is a gradual increase in levels of isoprostane from controls to OSMF to OSCC.
The results showed that the concentration of isoprostane in saliva showed a progressive and steady increase from control through OSMF to OSCC indicating that saliva could be used as an effective diagnostic tool in estimating tumor markers. Large scale studies correlating with other potentially malignant oral disorders are required to ascertain the role of 8-Isoprostane as an ideal tumor marker.
8-异前列腺素是由花生四烯酸脂质过氧化形成的稳定氧化应激标志物之一。它在所有生物体液中都有可检测到的量。在各种神经、心脏疾病和牙周疾病中都有8-异前列腺素升高的报道。
本研究旨在评估和比较口腔鳞状细胞癌（OSCC）、口腔黏膜下纤维化（OSMF）患者及对照组血浆和唾液中8-异前列腺素的水平。该研究还旨在确定8-异前列腺素是否可作为评估OSCC疾病进展的有效氧化应激标志物。
从10例临床诊断的OSMF患者、临床及组织病理学诊断的OSCC患者和对照组中分别采集血浆和唾液样本。对样本进行8-异前列腺素酶联免疫吸附测定（ELISA）程序并进行分析。使用SPSS软件进行统计分析。
血浆中8-异前列腺素水平从正常到OSMF再到OSCC呈平均升高趋势，但无统计学意义。唾液中8-异前列腺素水平的变化具有统计学意义（P = 0.037），表明从对照组到OSMF再到OSCC，异前列腺素水平逐渐升高。
结果表明，唾液中异前列腺素的浓度从对照组经OSMF到OSCC呈渐进性稳定升高，表明唾液可作为评估肿瘤标志物的有效诊断工具。需要进行与其他潜在恶性口腔疾病相关的大规模研究，以确定8-异前列腺素作为理想肿瘤标志物的作用。
