Liu Xiangjiang, Liang Yongjun, Lu Cuiyun, Chang Yumei, Li Chitao, Qin Xiangfeng, Quan Shuyue, Wang Zeyu, Zou Guiwei, Hu Guangfu
College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Huazhong Agricultural University, Wuhan, China.
Beijing Key Laboratory of fishery Biotechnology, Beijing Fisheries Research Institute, Beijing, China.
Mitochondrial DNA B Resour. 2016 Dec 9;1(1):925-926. doi: 10.1080/23802359.2016.1247661.
The complete mitogenomes of Ying hybrid carp (Russian scattered scale mirror carp ♀ × Carp-goldfish nucleocytoplasmic hybrid ♂) was sequenced by PCR-based method. The total of the mtDNA was 16,581 bp, and consisted of 13 protein-coding genes, 22 tRNAs, 2rRNA genes, and 1 control region (D-loop region). The overall base composition was as follows: A: 31.9%, T: 24.8%, C: 27.5%, and G: 15.8%, respectively. The overall AT and GC skew were found to be 0.125 and -0.271, respectively. A majority of the genes used ATG as the start codon, except that the codon GTG was found to initiated the gene. Most of the genes ended with the complete stop codon TAA or TAG, while the , , , and genes terminated with the incomplete stop codons 'T--'.
采用基于聚合酶链式反应（PCR）的方法对颖鲤（俄罗斯散鳞镜鲤♀×鲤-金鱼核质杂种♂）的完整线粒体基因组进行了测序。线粒体DNA总长为16,581bp，由13个蛋白质编码基因、22个tRNA、2个rRNA基因和1个控制区（D-loop区）组成。其总体碱基组成为：A占31.9%，T占24.8%，C占27.5%，G占15.8%。总体AT偏斜度和GC偏斜度分别为0.125和-0.271。大多数基因以ATG作为起始密码子，不过发现密码子GTG启动了该基因。大多数基因以完整的终止密码子TAA或TAG结束，而、、、和基因以不完整的终止密码子“T--”结束。
