沙蚕（环节动物门：沙蚕科）的完整线粒体基因组。
The complete mitogenome of nereid worm, (Annelida: Nereididae).
作者信息
Lin Geng-Ming, Audira Gilbert, Hsiao Chung-Der
机构信息
Laboratory of Marine Biology and Ecology, Third Institute of Oceanography, State Oceanic Administration, Xiamen, China.
Department of Bioscience Technology, Chung Yuan Christian University, Chung-Li, Taiwan.
出版信息
Mitochondrial DNA B Resour. 2017 Jul 31;2(2):471-472. doi: 10.1080/23802359.2017.1361346.
In this study, the complete mitogenome sequence of nereid worm, (Annelida: Nereididae), has been decoded for the first time by PCR amplification and Sanger sequencing methods. The overall base composition of mitogenome is 31.5% for A, 22.2% for C, 14.5% for G and 31.7% for T, and has GC content of 36.7%. The assembled mitogenome, consisting of 16,126 bp, has unique 13 protein-coding genes (PCGs), 22 transfer RNAs and 2 ribosomal RNAs genes. The complete mitogenome of shows 65% identities to . All PCGs, tRNA and rRNA genes were encoded on H-strand. The potential D-loop is 1625 bp in length and located between tRNA-Gly and tRNA-Met. The complete mitogenome provides essential and important DNA molecular data for further phylogenetic and evolutionary analysis for Annelida.
在本研究中，首次通过聚合酶链反应（PCR）扩增和桑格测序方法解码了沙蚕（环节动物门：沙蚕科）的完整线粒体基因组序列。该线粒体基因组的总体碱基组成为：腺嘌呤（A）占31.5%，胞嘧啶（C）占22.2%，鸟嘌呤（G）占14.5%，胸腺嘧啶（T）占31.7%，GC含量为36.7%。组装后的线粒体基因组由16,126个碱基对组成，包含独特的13个蛋白质编码基因（PCGs）、22个转运RNA和2个核糖体RNA基因。该沙蚕的完整线粒体基因组与[具体物种]具有65%的同一性。所有的PCGs、tRNA和rRNA基因均在重链上编码。潜在的控制区（D-loop）长度为1625个碱基对，位于tRNA-甘氨酸和tRNA-甲硫氨酸之间。完整的线粒体基因组为环节动物进一步的系统发育和进化分析提供了重要的DNA分子数据。
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