Sundquist B, Lövgren K, Morein B
National Veterinary Institute, Division of Vaccine Research, Uppsala, Sweden.
Vaccine. 1988 Feb;6(1):49-53. doi: 10.1016/0264-410x(88)90014-x.
A monovalent experimental ISCOM vaccine has been prepared with the envelope glycoproteins haemagglutinin and neuraminidase of the equine virus strain A/Solvalla/79 (H3N8). In vaccination trials on BALB/c mice the ISCOM vaccine induced more than ten times higher serum antibody titres measured in ELISA than a corresponding experimental micelle vaccine. Similarly, in guinea-pigs the ISCOMs induced about tenfold higher haemagglutination inhibition (HI) and neuraminidase inhibition (NI) titres than a micelle vaccine or a conventional killed influenza whole virus vaccine. Horses vaccinated with a divalent experimental ISCOM vaccine, containing the equine strains A/Prague/56 (H7N7) and A/Solvalla/79 (H3N8), responded with ELISA antibody titres against haemagglutinin which were higher and lasted considerably longer than those in horses vaccinated with conventional whole virus vaccine. ISCOMs induced complete immunoprotection in mice vaccinated with a dose of 1 microgram envelope glycoproteins of the mouse pathogenic strain A/PR/8/34 (H1N1).
一种单价实验性免疫刺激复合物（ISCOM）疫苗已用马病毒株A/Solvalla/79（H3N8）的包膜糖蛋白血凝素和神经氨酸酶制备而成。在对BALB/c小鼠的疫苗接种试验中，ELISA检测显示，ISCOM疫苗诱导产生的血清抗体滴度比相应的实验性胶束疫苗高出十多倍。同样，在豚鼠中，ISCOM诱导产生的血凝抑制（HI）和神经氨酸酶抑制（NI）滴度比胶束疫苗或传统的灭活流感全病毒疫苗高出约十倍。用含有马毒株A/Prague/56（H7N7）和A/Solvalla/79（H3N8）的二价实验性ISCOM疫苗接种马匹，ELISA检测显示，其针对血凝素的抗体滴度高于接种传统全病毒疫苗的马匹，且持续时间长得多。ISCOM对接种1微克小鼠致病株A/PR/8/34（H1N1）包膜糖蛋白的小鼠具有完全免疫保护作用。
