Li Xianghui, Hu Tongxin, Wei Jiacun, He Yuhua, Abdalla Abualgasim Elgaili, Wang Guoying, Li Yanzhang, Teng Tieshan
Institute of Biomedical Informatics, School of Basic Medical Sciences, Henan University, Kaifeng 475004, China.
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka 2014, Saudi Arabia.
Antibiotics (Basel). 2021 Feb 9;10(2):174. doi: 10.3390/antibiotics10020174.
phage Henu2 was isolated from a sewage sample collected in Kaifeng, China, in 2017. In this study, Henu2, a linear double-stranded DNA virus, was sequenced and found to be 43513bp long with 35% G + C content and 63 putative open reading frames (ORFs). Phage Henu2 belongs to the family and possesses an isometric head (63 nm in diameter). The latent time and burst size of Henu2 were approximately 20 min and 7.8 plaque forming unit (PFU)/infected cells. The Henu2 maintained infectivity over a wide range of temperature (10-60 °C) and pH values (4-12). Phylogenetic and comparative genomic analyses indicate that phage Henu2 should be a new member of the family of class-II. In this paper, Phage Henu2 alone exhibited weak inhibitory activity on the growth of . However, the combination of phage Henu2 and some antibiotics or oxides could effectively inhibit the growth of , with a decrease of more than three logs within 24 h in vitro. These results provide useful information that phage Henu2 can be combined with antibiotics to increase the production of phage Henu2 and thus enhance the efficacy of bacterial killing.
噬菌体Henu2于2017年从中国开封采集的一份污水样本中分离出来。在本研究中，对线性双链DNA病毒Henu2进行了测序，发现其长度为43513bp，G + C含量为35%，有63个推定的开放阅读框（ORF）。噬菌体Henu2属于该科，具有等轴状头部（直径63nm）。Henu2的潜伏期和裂解量分别约为20分钟和7.8个噬菌斑形成单位（PFU）/感染细胞。Henu2在较宽的温度范围（10 - 60°C）和pH值范围（4 - 12）内保持感染性。系统发育和比较基因组分析表明，噬菌体Henu2应是II类该科的一个新成员。在本文中，单独的噬菌体Henu2对[具体细菌名称缺失]的生长仅表现出微弱的抑制活性。然而，噬菌体Henu2与一些抗生素或氧化物的组合可有效抑制[具体细菌名称缺失]的生长，在体外24小时内细菌数量减少超过三个对数级。这些结果提供了有用信息，即噬菌体Henu2可与抗生素联合使用，以增加噬菌体Henu2的产量，从而提高杀菌效果。
