Department of Ophthalmology, Bucheon St. Mary's Hospital, College of Medicine, The Catholic University of Korea, #327 Sosa-ro, Wonmi-gu, Bucheon, Gyeonggi-do, 14647, Republic of Korea.
Int Ophthalmol. 2021 May;41(5):1845-1853. doi: 10.1007/s10792-021-01746-9. Epub 2021 Feb 20.
To investigate aqueous cytokine levels in association with hemorrhage in proliferative diabetic retinopathy (PDR) in patients with type 2 diabetes mellitus.
Sixty-six eyes with treatment-naïve PDR, including 26 hemorrhagic and 40 nonhemorrhagic eyes were included in this institutional study. Aqueous humor levels of interleukin (IL)-1b, IL-6, IL-8, IL-10, monocyte chemoattractant protein (MCP)-1, tumor necrosis factor (TNF)-α, vascular endothelial growth factor (VEGF), and soluble VEGF receptor-1 were obtained by multiplex bead assay. Visual acuity and hemorrhage area measurements were obtained, and correlations between cytokine levels and hemorrhage were identified.
Levels of MCP-1, TNF-α, and VEGF were higher in hemorrhagic eyes (1506.77 vs. 2131.31 pg/mL, 0.43 vs. 0.63 pg/mL, and 103.96 vs. 206.96 pg/mL; P = 0.050, 0.022, and 0.027, respectively). The levels of IL-8, MCP-1, TNF-α, and VEGF showed positive correlation with visual acuity (P = 0.019, 0.015, 0.001, and 0.014, respectively). The hemorrhage area revealed positive correlation with TNF-α and VEGF levels (P = 0.001 and < 0.001, respectively).
The presence and amount of hemorrhage in PDR were associated not only with VEGF concentration, but also with the levels of certain inflammatory cytokines, suggesting a role of both VEGF and inflammation in hemorrhagic eyes.
研究 2 型糖尿病增殖性糖尿病视网膜病变（PDR）患者房水中细胞因子与出血的关系。
本研究为单中心研究，纳入了 66 只未经治疗的 PDR 眼，包括 26 只出血眼和 40 只非出血眼。采用多指标微珠检测法检测房水中白细胞介素（IL）-1b、IL-6、IL-8、IL-10、单核细胞趋化蛋白（MCP）-1、肿瘤坏死因子（TNF）-α、血管内皮生长因子（VEGF）和可溶性 VEGF 受体-1 的水平。测量视力和出血面积，并确定细胞因子水平与出血之间的相关性。
出血眼中 MCP-1、TNF-α 和 VEGF 的水平较高（1506.77 比 2131.31 pg/ml，0.43 比 0.63 pg/ml，103.96 比 206.96 pg/ml；P=0.050、0.022 和 0.027）。IL-8、MCP-1、TNF-α 和 VEGF 水平与视力呈正相关（P=0.019、0.015、0.001 和 0.014）。出血面积与 TNF-α 和 VEGF 水平呈正相关（P=0.001 和 <0.001）。
PDR 中的出血及其程度不仅与 VEGF 浓度有关，还与某些炎症细胞因子的水平有关，这表明 VEGF 和炎症在出血眼中均起作用。
