Zhou Li, Wu Xing, Yang Fu, Zhang Minghao, Huang Rong, Liu Jikai
School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan 430074, China.
National Demonstration Center for Experimental Ethnopharmacology Education, South-Central University for Nationalities, Wuhan 430074, China.
Mar Drugs. 2021 Feb 25;19(3):124. doi: 10.3390/md19030124.
The phospholipids (PLs) from Antarctic krill oil were purified (>97.2%) using adsorption column chromatography. Forty-nine PL molecular species were characterized by ultrahigh-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF-MS). Most of molecular species contained eicosapentaenoic acid (EPA, 20:5), docosahexaenoic acid (DHA, 22:6), docosapentaenoic acid (DPA, 22:5), and arachidonic acid (AA, 20:4). Notably, a special species PC (20:5/22:6) (1298.17 nmol/g) and many ether PLs were detected. The Antarctic krill PL liposome (IC = 0.108 mg/mL) showed better anti-inflammatory activity than crude Antarctic krill oil (IC = 0.446 mg/mL). It could block NF-κB signaling pathway via suppression of IκB-α degradation and p65 activation and dose-dependently reduce the cellular content of inflammatory mediators including nitric oxide (NO), reactive oxygen species (ROS), and inflammatory cytokines in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. In addition, it can suppress carrageenan-induced mouse paw swelling. Results from the present study could provide a reference for better evaluation of nutritional and medicinal values of Antarctic krill oil.
采用吸附柱色谱法对南极磷虾油中的磷脂（PLs）进行纯化（>97.2%）。通过超高效液相色谱-四极杆-飞行时间质谱（UHPLC-Q-TOF-MS）对49种PL分子种类进行了表征。大多数分子种类含有二十碳五烯酸（EPA，20:5）、二十二碳六烯酸（DHA，22:6）、二十二碳五烯酸（DPA，22:5）和花生四烯酸（AA，20:4）。值得注意的是，检测到一种特殊的PC（20:5/22:6）种类（1298.17 nmol/g）和许多醚磷脂。南极磷虾PL脂质体（IC = 0.108 mg/mL）比粗制南极磷虾油（IC = 0.446 mg/mL）表现出更好的抗炎活性。它可以通过抑制IκB-α降解和p65激活来阻断NF-κB信号通路，并剂量依赖性地降低脂多糖（LPS）刺激的RAW 264.7细胞中包括一氧化氮（NO）、活性氧（ROS）和炎性细胞因子在内的炎性介质的细胞含量。此外，它可以抑制角叉菜胶诱导的小鼠爪肿胀。本研究结果可为更好地评估南极磷虾油的营养和药用价值提供参考。
