Thapa Punam, Lee Yoo Jin, Nguyen Tiep Tien, Piao Donglan, Lee Hwaryeong, Han Sujin, Lee Yeon Jin, Han Ah-Reum, Choi Hyukjae, Jeong Jee-Heon, Nam Joo-Won, Seo Eun Kyoung
College of Pharmacy, Yeungnam University, Gyeongsan, Gyeongsangbukdo 38541, Korea.
College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul 03760, Korea.
Molecules. 2021 Mar 21;26(6):1762. doi: 10.3390/molecules26061762.
Miquel (Zingiberaceae) has been reported to show antioxidant, anti-inflammatory, and neuroprotective effects. In this study, two new eudesmane sesquiterpenes, 7α-hydroperoxy eudesma-3,11-diene-2-one () and 7β-hydroperoxy eudesma-3,11-diene-2-one (), and a new eremophilane sesquiterpene, 3α-hydroxynootkatone (), were isolated from the MeOH extract of dried fruits of along with eleven known sesquiterpenes (-). The structures were elucidated by the analysis of 1D/2D NMR, high-resolution electrospray ionization mass spectrometry (HRESIMS), and optical rotation data. Compounds (-, -) were evaluated for their protective effects against -butyl hydroperoxide (tBHP)-induced oxidative stress in adipose-derived mesenchymal stem cells (ADMSCs). As a result, treatment with isolated compounds, especially compounds and effectively reverted the damage of tBHP on ADMSCs in a dose-dependent manner. In particular, and at 50 µM improved the viability of tBHP-toxified ADMSCs by 1.69 ± 0.05-fold and 1.61 ± 0.03-fold, respectively.
据报道，姜科植物米格尔（Miquel）具有抗氧化、抗炎和神经保护作用。在本研究中，从米格尔干燥果实的甲醇提取物中分离出两种新的桉叶烷倍半萜，7α-氢过氧化桉叶-3,11-二烯-2-酮（）和7β-氢过氧化桉叶-3,11-二烯-2-酮（），以及一种新的苦艾烷倍半萜，3α-羟基诺卡酮（），同时还分离出11种已知的倍半萜（-）。通过1D/2D NMR、高分辨率电喷雾电离质谱（HRESIMS）和旋光数据对结构进行了阐明。评估了化合物（-，-）对叔丁基过氧化氢（tBHP）诱导的脂肪来源间充质干细胞（ADMSCs）氧化应激的保护作用。结果，用分离出的化合物处理，尤其是化合物和能以剂量依赖的方式有效逆转tBHP对ADMSCs的损伤。特别是，50μM的和分别使tBHP毒性化的ADMSCs的活力提高了1.69±0.05倍和1.61±0.03倍。
