Eudesmane and Eremophilane Sesquiterpenes from the Fruits of with Protective Effects against Oxidative Stress in Adipose-Derived Mesenchymal Stem Cells.

Miquel (Zingiberaceae) has been reported to show antioxidant, anti-inflammatory, and neuroprotective effects. In this study, two new eudesmane sesquiterpenes, 7α-hydroperoxy eudesma-3,11-diene-2-one () and 7β-hydroperoxy eudesma-3,11-diene-2-one (), and a new eremophilane sesquiterpene, 3α-hydroxynootkatone (), were isolated from the MeOH extract of dried fruits of along with eleven known sesquiterpenes (-). The structures were elucidated by the analysis of 1D/2D NMR, high-resolution electrospray ionization mass spectrometry (HRESIMS), and optical rotation data. Compounds (-, -) were evaluated for their protective effects against -butyl hydroperoxide (tBHP)-induced oxidative stress in adipose-derived mesenchymal stem cells (ADMSCs). As a result, treatment with isolated compounds, especially compounds and effectively reverted the damage of tBHP on ADMSCs in a dose-dependent manner. In particular, and at 50 µM improved the viability of tBHP-toxified ADMSCs by 1.69 ± 0.05-fold and 1.61 ± 0.03-fold, respectively.